Electronic Detection Immunoassays that Utilize a Binder Support Medium
First Claim
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1. A device comprising:
- a) a binder support medium comprising at least one detection zone for the detection of an analyte in a liquid sample;
b) at least one label moiety, wherein the label is capable of participating in the generation of a dopant;
c) a conducting polymer in fluid communication with the binder support medium, the conducting polymer capable of having its electrical conductivity changed after being doped with the dopant;
d) a circuit that comprises a voltage source, wherein an electric potential may be maintained across at least one portion of one dimension of the conducting polymer by means of the circuit.
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Abstract
A binder support medium-based immunoassay device and method is provided, utilizing the catalyzed formation of dopants and their subsequent effects on electroconductive polymers to detect an analyte of interest.
43 Citations
32 Claims
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1. A device comprising:
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a) a binder support medium comprising at least one detection zone for the detection of an analyte in a liquid sample;
b) at least one label moiety, wherein the label is capable of participating in the generation of a dopant;
c) a conducting polymer in fluid communication with the binder support medium, the conducting polymer capable of having its electrical conductivity changed after being doped with the dopant;
d) a circuit that comprises a voltage source, wherein an electric potential may be maintained across at least one portion of one dimension of the conducting polymer by means of the circuit. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A device comprising:
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a) a binder support medium comprising at least one detection zone for the detection of an analyte in a liquid sample;
b) at least one chemical compound that is in fluid communication with the binder support medium, and which is capable of being transformed into a dopant or a dopant precursor compound by a reduction-oxidation enzyme;
c) at least one label moiety, wherein the label moiety is a reduction-oxidation enzyme capable of participating in the generation of the dopant;
d) a conducting polymer component in fluid communication with the binder support medium, the conducting polymer component capable of having its electrical conductivity changed after being doped with the dopant; and
,e) a circuit that comprises a voltage source, wherein an electric potential may be maintained across at least one portion of one dimension of the conducting polymer by means of the circuit. - View Dependent Claims (13, 14, 15, 16, 17, 18)
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19. A method for detecting the presence or amount of an analyte in a liquid sample comprising:
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a) applying a liquid sample suspected of containing an analyte to a device;
b) contacting the analyte, if present, with a labeled receptor molecule, wherein the label is a reduction-oxidation enzyme, to form an analyte-labeled receptor complex;
c) transporting the analyte-labeled receptor complex through a binder support medium by means of liquid flow to a detection zone portion of the binder support medium;
d) immobilizing the analyte-labeled receptor complex in the detection zone portion of the binder support medium;
e) removing by liquid flow any reduction-oxidation enzyme that is not immobilized in the detection zone portion of the binder support medium, wherein the removal by liquid flow may occur prior to or concurrent with any of the events below;
f) transforming at least one chemical compound by the reduction-oxidation enzyme portion of the immobilized analyte-labeled receptor complex to form a dopant;
g) diffusing the dopant into or onto the surface of a conducting polymer that is in fluid communication with the detection zone portion, to form a doped conducting polymer; and
,h) detecting the degree of doping of the conducting polymer by applying an electrical potential across the conducting polymer, to determine the presence, the amount, or the presence and amount of the analyte. - View Dependent Claims (20, 21, 22)
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23. A method for detecting the presence or amount of an analyte in a liquid sample comprising:
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a) applying a liquid sample suspected of containing an analyte to a device;
b) contacting the analyte, if present, with a labeled receptor molecule, wherein the label is a first reduction-oxidation enzyme, to form an analyte-labeled receptor complex;
c) transporting the analyte-labeled receptor complex through a binder support medium by means of liquid flow to a detection zone portion of the binder support medium;
d) immobilizing the analyte-labeled receptor complex in the detection zone portion of the binder support medium;
e) removing by liquid flow any first reduction-oxidation enzyme that is not immobilized in the detection zone portion of the binder support medium, wherein the removal by liquid flow may occur prior to or concurrent with any of the events below;
f) transforming at least one chemical compound with the first reduction-oxidation enzyme portion of the immobilized analyte-labeled receptor complex, to form a dopant precursor molecule;
g) transforming the dopant precursor molecule to a dopant with a second reduction-oxidation enzyme that is present within the detection zone portion;
h) diffusing the dopant into or onto the surface of a conducting polymer that is in fluid communication with the detection zone portion, to form a doped conducting polymer; and
i) detecting the degree of doping of the conducting polymer by applying an electrical potential across the conducting polymer, to determine the presence, the amount, or both the presence and amount of the analyte. - View Dependent Claims (24, 25, 26)
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27. A method for detecting the presence or amount of an analyte in a liquid sample comprising the steps of:
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a) applying a liquid sample containing or lacking an analyte to a device, wherein the device comprises a competitor bound to a label moiety forming a competitor complex, the competitor being an analyte, analogue, or derivative thereof, and the label moiety being a reduction-oxidation enzyme;
b) transporting the competitor complex and analyte contained in the liquid sample, if present, through a binder support medium by means of liquid flow to a detection zone portion of the binder support medium;
c) immobilizing the analyte in the detection zone whereby the competitor portion of the competitor complex and the analyte contained in the liquid sample compete for the binding of the binder in the detection zone by means of a ligand-receptor interaction;
d) removing by liquid flow any competitor complex that is not immobilized in the detection zone, wherein the removal by liquid flow may occur prior to or concurrent with any of the events below;
e) transforming at least one chemical compound with the reduction-oxidation enzyme portion of the immobilized competitor complex to form a dopant;
f) diffusing the dopant into or onto the surface of a conducting polymer that is in fluid communication with the detection zone portion, to form a doped conducting polymer; and
g) detecting the degree of doping of the conducting polymer by applying an electrical potential across the conducting polymer, to determine the amount, the presence or the presence and amount of the analyte. - View Dependent Claims (28, 29)
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30. A method for detecting the presence or amount of an analyte in a liquid sample comprising:
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a) applying a liquid sample containing or lacking an analyte to a device, wherein the device contains a competitor bound to a label forming a competitor complex, the competitor being an analyte, analogue, or derivative thereof, and the label being a reduction-oxidation enzyme;
b) transporting the competitor complex and analyte contained in the liquid sample, if present, through a binder support medium by means of liquid flow to a detection zone portion of the binder support medium;
c) immobilizing the analyte in the detection zone whereby the competitor portion of the competitor complex and the analyte contained in the liquid sample compete for the binding of the binder in the detection zone by means of a ligand-receptor interaction;
d) removing by liquid flow any competitor complex that is not immobilized in the detection zone, wherein the removal by liquid flow may occur prior to or concurrent with any of the events below;
e) transforming at least one chemical compound with the reduction-oxidation enzyme portion of the immobilized competitor complex to form a dopant precursor molecule;
f) transforming the dopant precursor molecule to a dopant with a second reduction-oxidation enzyme that is present within the detection zone portion;
g) diffusing the dopant into or onto the surface of a conducting polymer that is in fluid communication with the detection zone portion, to form a doped conducting polymer; and
h) detecting the degree of doping of the conducting polymer by applying an electrical potential across the conducting polymer component, to determine the presence, the amount or the presence and amount of the analyte. - View Dependent Claims (31, 32)
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Specification