Process for producing normal parenchymal cells, tissues or organs by bioincubator
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Abstract
Methods of producing cells, tissues and organs as close as possible to homoplasy have been developed to provide nonhuman mammals having normal parenchymal cells of a foreign mammal, a tissue made up of normal cells of a foreign mammal and their secretion products thereof, and/or an organ of a foreign mammal.
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Citations
25 Claims
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1-14. -14. (canceled)
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15. A method for promoting the production of foreign-derived mammalian cells of preselected parenchymal cell types in a non-human mammal host, comprising the steps of:
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providing a fetal non-human mammal host comprising a DNA element in its genome that comprises a promoter region functioning specifically in a preselected parenchymal cell type of an organ or tissue and a structural gene encoding an active cytotoxin or an enzyme to activate a cytotoxin precursor, under control of said promoter region so that endogenous host cells of the preselected parenchymal cell type express the cytotoxin or the enzyme;
adding foreign mammalian cells of a different species than the host to the host to form a chimeric host, wherein the foreign mammalian cells do not express a gene for an active cytotoxin and do not express a gene for an enzyme capable of converting the cytotoxin precursor into the cytotoxin and wherein the foreign mammalian cells are capable of proliferating into or differentiating into cells of the preselected parenchymal cell type;
allowing the chimeric host to develop while the endogenous host cells of the preselected parenchymal cell type formed in the course of the development of the host specifically express the active cytotoxin or the cytotoxin precursor activating enzyme, and in the case where the endogenous host cells of the preselected parenchymal cell type express the cytotoxin precursor activating enzyme, adding the cytotoxin precursor to the host, wherein the development of foreign mammalian cell-derived cells of the preselected parenchymal cell type is promoted in the host. - View Dependent Claims (16, 17, 18, 19, 20)
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21. A method for promoting the proliferation/differentiation of foreign mammalian cells in a living non-human mammal host into cells or a tissue or organ while removing specific endogenous cells of the host, which comprises the steps of:
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providing a living non-human mammalian host wherein cells of a preselected cell type of an organ or tissue selectively express a gene for an enzyme capable of converting a cytotoxin precursor into a cytotoxin;
adding foreign mammalian cells to the host, wherein the foreign mammalian cells do not express a gene for an enzyme capable of converting the cytotoxin precursor into the cytotoxin and wherein the foreign mammalian cells are capable of proliferating into or differentiating into cells of the preselected cell type; and
adding a cytotoxin precursor into the host to specifically remove the endogenous cells of the host of the preselected cell type in which the gene for the enzyme capable of converting the cytotoxin precursor into the cytotoxin is expressed, wherein the development of foreign mammalian cell-derived cells of the preselected cell type is promoted in the host. - View Dependent Claims (22, 23, 24, 25)
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Specification