Method of detecting biosensor filling
First Claim
1. A method of detecting application of liquid sample to a biosensor comprising contacting the liquid sample with a biosensor comprising an opening to a capillary channel containing a first reference electrode, a working electrode, and a second reference electrode comprised on a substrate, electrically connecting the biosensor to a device for applying voltage to the biosensor;
- applying a first voltage (V1) to the working electrode relative to the first reference electrode and detecting a first current (i1);
applying a second voltage (V2) to the working electrode relative to the second reference electrode and detecting a second current (i2), wherein the presence of i2 indicates sample has been applied to the biosensor;
thereby detecting application of the liquid sample to the biosensor.
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Abstract
The present invention is directed to methods of detecting filling of the capillary channel of a biosensor test strip. In one embodiment the biosensor test strip has three electrodes: a first reference electrode, a working electrode and a second reference electrode, which are arranged sequentially within the capillary channel of a biosensor test strip and perpendicular to the axis thereof. A test sample containing an analyte of interest is applied to the test strip. A first voltage (V1) is applied between the first reference electrode and the working electrode, and a first current (i1) is detected. If i1 is detected, a second voltage (V2) is applied between the working electrode and the second reference electrode. A second current (i2) is detected, indicating that an applied sample has covered the first and second reference electrodes and the working electrodes. The measuring device then conducts the assay for the presence or amount of the analyte of interest in the test sample.
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Citations
21 Claims
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1. A method of detecting application of liquid sample to a biosensor comprising
contacting the liquid sample with a biosensor comprising an opening to a capillary channel containing a first reference electrode, a working electrode, and a second reference electrode comprised on a substrate, electrically connecting the biosensor to a device for applying voltage to the biosensor; -
applying a first voltage (V1) to the working electrode relative to the first reference electrode and detecting a first current (i1);
applying a second voltage (V2) to the working electrode relative to the second reference electrode and detecting a second current (i2), wherein the presence of i2 indicates sample has been applied to the biosensor;
thereby detecting application of the liquid sample to the biosensor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification