Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
First Claim
Patent Images
1. An analytical device, comprising:
- a substrate;
a plurality of signal sources disposed upon the substrate, the signal sources being arrayed upon the substrate in a plurality of substantially parallel rows, each of the plurality of parallel rows comprising a plurality of signal sources, and wherein two adjacent signal sources in a row are spaced apart by a first distance, and wherein two adjacent rows of signal sources are spaced apart by a second distance, wherein the second distance is greater than the first distance.
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Abstract
Methods and systems for real-time monitoring of optical signals from arrays of signal sources, and particularly optical signal sources that have spectrally different signal components. Systems include signal source arrays in optical communication with optical trains that direct excitation radiation to and emitted signals from such arrays and image the signals onto detector arrays, from which such signals may be subjected to additional processing.
203 Citations
64 Claims
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1. An analytical device, comprising:
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a substrate;
a plurality of signal sources disposed upon the substrate, the signal sources being arrayed upon the substrate in a plurality of substantially parallel rows, each of the plurality of parallel rows comprising a plurality of signal sources, and wherein two adjacent signal sources in a row are spaced apart by a first distance, and wherein two adjacent rows of signal sources are spaced apart by a second distance, wherein the second distance is greater than the first distance. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. An array of signal sources on a substrate, the array comprising:
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a first plurality of signal sources located at a first location on the substrate, wherein a first signal source of the first plurality of signal sources is a first distance from a second signal source in the first plurality of signal sources; and
a second plurality of signal sources located at a second location on the substrate, wherein a first signal source in the second plurality of signal sources is a second distance from a second signal source in the second plurality of signal sources, the second distance being greater than the first distance. - View Dependent Claims (16, 17, 18, 19, 20)
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21. An analytical system, comprising:
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a substrate having a plurality of discrete signal sources disposed thereon;
an excitation light source;
an optical train positioned to transmit excitation light from the excitation light source to the substrate to illuminate a first plurality of illuminated signal sources and a second plurality of illuminated signal sources, and image signals from the plurality of illuminated signal sources onto an array detector; and
wherein one or more of the substrate and the optical train are configured such that the first plurality of illuminated signal sources is spaced from the second plurality of illuminated signal sources by a first distance that is greater than a cross sectional dimension of an image of a signal from a signal source in the first plurality of signal sources that is imaged onto the array detector. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30)
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31. A system, comprising:
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an optical train for receiving signals from an image field on an array of signal sources and imaging said signals onto a detector; and
an array of signal sources, wherein a distance between two adjacent signal sources at a periphery of the image field is greater than a distance between two adjacent signal sources at a center of the image field on the array. - View Dependent Claims (38, 39, 40)
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32. An analytical system, comprising:
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a substrate having a plurality of signal sources disposed thereon;
an excitation light source;
an optical train configured to;
receive excitation light from the excitation light source;
direct the excitation light onto the substrate in at least first and second substantially parallel linear illumination profiles, wherein the first and second linear illumination profiles are spaced apart on the substrate by a distance that is greater than a width of the first linear illumination profile; and
receive optical signals from the substrate and image the optical signals onto a detector array. - View Dependent Claims (33, 34, 35, 36, 37)
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41. An analytical system, comprising:
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a substrate comprising a first and second signal source separated by a first space;
an excitation light source; and
an optical train configured to direct excitation light from the excitation light source to the first and second signal source simultaneously, without directing excitation light at at least a portion of the first space. - View Dependent Claims (42, 43)
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44. An analytical system, comprising:
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a substrate comprising a plurality of discrete signal sources disposed thereon, wherein at least a first subset of signal sources are positioned in a first substantial linear orientation, and a second subset of signal sources are positioned in a second substantially linear orientation that is substantially parallel to the first linear orientation;
a light source;
an optical train for directing light from the light source to the substrate in at least first and second substantially parallel linear illumination profiles, the first linear profile illuminating the first subset of signal sources and the second illumination profile illuminating the second subset of signal sources. - View Dependent Claims (45, 46)
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47. A system, comprising:
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a substrate comprising a plurality of discrete signal sources;
an excitation light source;
an optical train, positioned to receive excitation light from the excitation light source and direct the excitation light to the substrate, wherein the optical train is configured to direct excitation light in a substantially linear illumination profile at a plurality of signal sources, simultaneously, and simultaneously receive optical signals from the plurality of signal sources and direct the optical signals upon an imaging detector, to detect the optical signals from the plurality of signal sources.
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48. A system, comprising:
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a substrate having at least first and second rows of signal sources disposed thereon;
an excitation light source; and
an optical train, positioned to receive excitation light from the excitation light source and direct the excitation light to the substrate, wherein the optical train is configured to divide the excitation light into at least first and second discrete beams, and direct each of the at least first and second discrete beams in a substantially linear illumination profile at the substrate, wherein the first beam simultaneously illuminates a plurality of signal sources in the first row of signal sources, and the second beam simultaneously illuminates a plurality of signal sources in the second row of signal sources.
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49. A system, comprising:
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an excitation light source;
an optical train positioned to receive excitation light from the excitation light source and direct excitation light to the substrate, the optical train comprising;
a polarizing beam splitter to split the excitation light into at least first and second polar component beams; and
optical components for directing each of the first and second polar component beams to different locations on the substrate.
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50. A method of analyzing a plurality of signal sources, comprising:
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providing a substrate having a plurality of discrete signal sources disposed thereon;
illuminating a first plurality of illuminated signal sources and a second plurality of illuminated signal sources, wherein the first plurality of illuminated signal sources are spaced apart from the second plurality of illuminated signal sources by a distance that is greater than a cross sectional dimension of an image of the signal source imaged on a detector array; and
imaging the signal source on a detector array. - View Dependent Claims (51, 52, 53, 54, 55, 56, 57)
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58. A method of analyzing a plurality of signal sources, comprising:
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providing a substrate having a plurality of discrete signal sources disposed thereon and oriented in at least a first and second rows of signal sources, wherein the first and second rows of signal sources are spaced apart by a first distance;
directing excitation light in at least first and second linear illumination profiles at the first and second rows of signal sources, respectively;
wherein the first distance is greater than a width of the first linear illumination profiles; and
analyzing signals from the plurality of discrete signal sources in response to the excitation light. - View Dependent Claims (59, 60)
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61. A method of detecting fluorescent signals from a plurality of signal sources on a substrate, comprising directing excitation radiation at portions of the substrate occupied the plurality of signal sources on a substrate while not directing excitation radiation at portions of the substrate not occupied by the signal sources.
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62. A method of analyzing a plurality of signal sources on a substrate, comprising:
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providing at least first and second adjacent signal sources on a substrate;
selectively directing excitation radiation at the first and second signal sources while not substantially illuminating space between the first and second signal sources. - View Dependent Claims (63, 64)
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Specification