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KITS USEFUL FOR SEQUENCING NUCLEIC ACIDS

  • US 20070207477A1
  • Filed: 12/29/2006
  • Published: 09/06/2007
  • Est. Priority Date: 05/03/1996
  • Status: Active Grant
First Claim
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1. A kit useful for sequencing a nucleic acid, comprising a set of four different terminating nucleotides, each of which terminates a template-dependent primer extension reaction at a different template nucleotide and comprises a fluorescent label capable of producing a detectable, spectrally resolvable fluorescent signal, wherein at least one of the fluorescent labels is an energy transfer dye comprising:

  • a xanthene donor dye capable of absorbing light at a first wavelength and emitting excitation energy in response thereto;

    a 4,7-dichlororhodamine acceptor dye capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response thereto; and

    a non-nucleosidic linker linking the xanthene donor dye to the 4,7-dichlororodamine acceptor dye.

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