Design and construction of dimeric concanavalin a mutants
First Claim
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1. A purified mutant Concanavalin A (ConA) protein comprising the amino acid sequence of SEQ ID NO:
- 16, wherein said sequence comprises a substitution at amino acid residue 58 and a substitution at one or more of amino acid residue 118, amino acid residue 121, and amino acid residue 192, said purified mutant Con A having reduced dimer-dimer affinity compared to a corresponding wild type ConA protein.
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Abstract
Embodiments of the invention provide for compositions comprising purified polypeptides such as purified Concanavalin A (ConA) mutants. In addition, embodiments provide for polypeptides and nucleic acids encoding those polypeptides, such as mutant ConA with reduced dimer-dimer interactions compared to wild type ConA. Some embodiments also provide for sensors comprising the polypeptides disclosed herein. The embodiments also provide an improved method of producing recombinant mutant ConA.
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27 Claims
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1. A purified mutant Concanavalin A (ConA) protein comprising the amino acid sequence of SEQ ID NO:
- 16, wherein said sequence comprises a substitution at amino acid residue 58 and a substitution at one or more of amino acid residue 118, amino acid residue 121, and amino acid residue 192, said purified mutant Con A having reduced dimer-dimer affinity compared to a corresponding wild type ConA protein.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 23, 24, 25, 26, 27)
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21. A purified mutant Concanavalin A (Con A) molecule, wherein the molecule comprises a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 6, 8, 10, 12, 14, 18, 20, 22, 24, and 26, or biologically active variants thereof.
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22. A purified, isolated nucleic acid selected from the group consisting of SEQ ID NOs:
- 5, 7, 9, 11, 13, 17, 19, 21, 23, and 25.
Specification