Methods, Products, and Kits for Identifying an Analyte in a Sample
First Claim
1. A method of performing an assay to identify an analyte in a sample, comprising:
- combining the sample with a set of beads, at least some of which are adapted to couple to the analyte in the sample;
mixing an enzyme with the sample and bead combination, the enzyme being adapted to couple to the analyte;
interacting a substrate with the enzyme where a property of the substrate is modified by the enzyme and the modified substrate is attracted to the beads; and
illuminating the beads to identify modified substrate attracted to the beads.
1 Assignment
0 Petitions
Accused Products
Abstract
Methods, products, and kits for identifying an analyte in a sample are provided. One embodiment of a method for identifying an analyte in a sample includes combining the sample with a first reactant capable of specifically coupling to the analyte. The first reactant is coupled to beads. The method also includes combining additional reactant with the beads. The additional reactant is capable of specifically coupling to the analyte or a second reactant coupled to an analyte. An enzyme is attached to the additional reactant. In addition, the method includes combining a substrate with the beads. The substrate is capable of specifically interacting with the enzyme to form a modified substrate. If the substrate interacts with the enzyme attached to the beads via the additional reactant, the solubility of the substrate changes causing the modified substrate to bind to a surface of the beads and/or the reactants bound to the beads. The method further includes identifying the analyte in the sample by detecting the modified substrate bound to the surface of the beads and/or the reactants bound to the beads.
-
Citations
20 Claims
-
1. A method of performing an assay to identify an analyte in a sample, comprising:
-
combining the sample with a set of beads, at least some of which are adapted to couple to the analyte in the sample; mixing an enzyme with the sample and bead combination, the enzyme being adapted to couple to the analyte; interacting a substrate with the enzyme where a property of the substrate is modified by the enzyme and the modified substrate is attracted to the beads; and illuminating the beads to identify modified substrate attracted to the beads. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
-
-
11. A method for identifying an analyte in a sample, comprising:
-
combining the sample with a first reactant capable of coupling to the analyte, wherein the first reactant is coupled to beads; mixing an additional reactant with the beads, wherein the additional reactant is capable of coupling to the analyte, and wherein an enzyme is attached to the additional reactant; combining a substrate with the beads and reactant and enzyme mixture, wherein the substrate is capable of interacting with the enzyme to form a modified substrate wherein the solubility of the modified substrate changes causing the modified substrate to bind to a surface of the beads and/or the reactants bound to the beads; and identifying the analyte in the sample by detecting the bound modified substrate. - View Dependent Claims (12, 13)
-
-
14. A kit configured for use in flow cytometry for identifying an analyte in a sample, comprising:
-
a first reactant capable of specifically coupling to the analyte, wherein the first reactant is coupled to beads; a second reactant capable of specifically coupling to the analyte, the second reactant including an enzyme attached to the second reactant; and a substrate capable of specifically interacting with the enzyme to form a modified substrate, wherein if the substrate interacts with the enzyme the solubility of the substrate changes and the modified substrate will be attracted to the surface of the beads. - View Dependent Claims (15, 16, 17)
-
-
18. A product of a method for identifying an analyte in a sample by flow cytometry, comprising:
-
a first reactant coupled to the analyte, wherein the first reactant is further coupled to a bead; a second reactant coupled to the analyte wherein an enzyme is attached to the second reactant; and a modified substrate attracted to a surface of the bead and/or the reactants bound to the bead due to an interaction between an initial substrate and the enzyme that produced a change of the initial substrate. - View Dependent Claims (19, 20)
-
Specification