Phytase enzymes, nucleic acid sequences encoding phytase enzymes and vectors and host cells incorporating same
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Abstract
DNA is provided which encodes an enzyme having phytase activity isolated from Penicillium, Fusarium, Humicola and Emericella. Also provided for is a method of isolating DNA encoding an enzyme having phytase activity from organisms which possess such DNA, transformation of the DNA into a suitable host organism, expression of the transformed DNA and the use of the expressed phytase protein in feed as a supplement.
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Citations
31 Claims
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1-12. -12. (canceled)
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13. An isolated polynucleotide encoding an enzyme, said enzyme comprising an amino acid sequence having at least 55% identity to an amino acid sequence as disclosed in
FIG. 5, 6 , or 18A-18C (SEQ ID NO:- 5, 6 or 42, respectively).
- View Dependent Claims (15, 16, 17, 19, 20, 21, 25)
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14. An isolated polynucleotide including a nucleotide sequence (i) having at least 55% identity to a nucleotide sequence as disclosed in
FIG. 4 , or 18A-18C (SEQ ID NO:- 4 and 41, respectively), or (ii) being capable of hybridizing to a probe derived from the nucleotide sequence disclosed in
FIG. 4 , l or 18A-18C (SEQ ID NO;
4 and 41, respectively) under conditions of intermediate to high stringency, or (iii) being complementary to a nucleotide sequence having at least 55% identity to a nucleotide sequence as disclosed inFIG. 4 , or 18A-18C (SEQ ID NO;
4 and 41, respectively). - View Dependent Claims (26, 27, 28, 29, 30, 31)
- 4 and 41, respectively), or (ii) being capable of hybridizing to a probe derived from the nucleotide sequence disclosed in
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18. Food or animal feed including an enzyme having phytase activity, wherein said enzyme includes an amino acid sequence having at least 55% identity to an amino acid sequence as disclosed in
FIG. 5, 6 , or 18A-18C (SEQ ID NO:- 5, 6 and 42, respectively).
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22. A method of separating phosphorous from phytate, comprising:
- treating said phytate with an enzyme (i) having phytate hydrolyzing activity and (ii) including an amino acid sequence having at least 55% identity to an amino acid sequence as disclosed in
FIG. 5, 6 , or 18A-18C (SEQ ID NO;
5, 6 and 42, respectively).
- treating said phytate with an enzyme (i) having phytate hydrolyzing activity and (ii) including an amino acid sequence having at least 55% identity to an amino acid sequence as disclosed in
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23. An isolated polynucleotide encoding an enzyme having phytase activity, wherein said enzyme includes an amino acid sequence having at least 55% amino acid sequence identity to SEQ ID NO:
- 5, 6 or 42.
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24. Food or animal feed including an enzyme having phytase activity, wherein said enzyme comprises an amino acid sequence having at least 55% amino acid sequence identity to SEQ ID NO:
- 5, 6 or 42.
Specification