Phytase enzymes, nucleic acid sequences encoding phytase enzymes and vectors and host cells incorporating same

• US 20070207525A1
• Filed: 01/26/2007
• Published: 09/06/2007
• Est. Priority Date: 10/26/2001
• Status: Active Grant

• Current Assignee: Benjamin Bower, Frits Goedegebuur, Kathleen Clarkson, Power Scott
• Original Assignee: Benjamin Bower, Frits Goedegebuur, Kathleen Clarkson, Power Scott
• Inventors: Goedegebuur, Frits, Bower, Benjamin, Clarkson, Kathleen, Power, Scott
• Granted Patent: US 7,449,317 B2
• Time in Patent Office: Days
• US Class Current: 435/69.100
• CPC Class Codes: C12N 9/16 acting on ester bonds (3.1)

Abstract

DNA is provided which encodes an enzyme having phytase activity isolated from Penicillium, Fusarium, Humicola and Emericella. Also provided for is a method of isolating DNA encoding an enzyme having phytase activity from organisms which possess such DNA, transformation of the DNA into a suitable host organism, expression of the transformed DNA and the use of the expressed phytase protein in feed as a supplement.

Claims

1-12. -12. (canceled)

13. An isolated polynucleotide encoding an enzyme, said enzyme an amino acid sequence having at least 55% identity to an amino acid sequence as disclosed in FIG. 8 or 21 (SEQ ID NO:

8).

15. An expression construct including a polynucleotide according to claim 13.

16. A vector including the expression construct of claim 15.

17. A host cell transformed with the vector of claim 16.

19. A method of producing an enzyme having phytase activity, comprising:

(a) providing a host cell transformed with an expression vector comprising a polynucleotide as defined in claim 13;

(b) cultivating said transformed host cell under conditions suitable for said host cell to produce said phytase; and

(c) recovering said phytase.

20. The method of claim 19, wherein said host cell is an Aspergillus species.

21. A purified enzyme having phytase activity, produced by the method of claim 20.

25. The method according to claim 19, wherein the polynucleotide encodes an enzyme having phytase activity and at least 55% sequence identity to SEQ ID NO:

8.

14. An isolated polynucleotide including a nucleotide sequence (i) having at least 55% identity to a nucleotide sequence as disclosed in FIG. 7, and 21 (SEQ ID NO:

7), or (ii) being capable of hybridizing to a probe derived from the nucleotide sequence disclosed in FIGS. 7 and 21 (SEQ ID NO;

7) under conditions of intermediate to high stringency, or (iii) being complementary to a nucleotide sequence having at least 55% identity to a nucleotide sequence as disclosed in FIG. 7 and 21 (SEQ ID NO;

7).

26. An expression construct including a polynucleotide according to claim 14.

27. A vector including the expression construct of claim 26.

28. A host cell transformed with the vector of claim 27.

29. A method of producing an enzyme having phytase activity, comprising:

(a) providing a host cell transformed with an expression vector comprising a polynucleotide as defined in claim 14;

(b) cultivating said transformed host cell under conditions suitable for said host cell to produce said phytase; and

(c) recovering said phytase.

30. The method of claim 29, wherein said host cell is an Aspergillus species.

31. A purified enzyme having phytase activity, produced by the method of claim 30.

18. Food or animal feed including an enzyme having phytase activity, wherein said enzyme includes an amino acid sequence having at least 55% identity to an amino acid sequence as disclosed in FIG. 8 or 21 (SEQ ID NO:

8).

22. A method of separating phosphorous from phytate, comprising:

treating said phytate with an enzyme (i) having phytate hydrolyzing activity and (ii) including an amino acid sequence having at least 55% identity to an amino acid sequence as disclosed in FIG. 8 or 21 (SEQ ID NO;

8).

23. An isolated polynucleotide encoding an enzyme having phytase activity, wherein said enzyme includes an amino acid sequence having at least 55% amino acid sequence identity to SEQ ID NO:

8.

24. Food or animal feed including an enzyme having phytase activity, wherein said enzyme comprises an amino acid sequence having at least 55% amino acid sequence identity to SEQ ID NO:

8.