Ultra-sensitive detection systems using multidimension signals
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Abstract
Disclosed are compositions and methods for sensitive detection of one or multiple analytes. In general, the methods involve the use of special label components, referred to as multidimension signals. In the disclosed methods, analysis of multidimension signals can result in one or more predetermined patterns that serve to indicate whether a further level of analysis can or should be performed and/or which portion(s) of the analyzed material can or should be analyzed in a further level of analysis. In some forms, isobaric and non-isobaric elements can be used together in the same assay or assay system. Isobaric and non-isobaric multidimension signals used together can generate one or more predetermined patterns during analysis. The pattern generated in this first level of analysis indicates whether the second level of analysis should be performed. The second level of analysis can involve distinguishing the isobaric multidimension signals.
71 Citations
110 Claims
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1-33. -33. (canceled)
- 34. A reporter signal peptide comprising the amino acid sequence Gly-Gly-Gly-Gly-Gly-Gly-Asp-Pro-Gly-Gly-Gly-Gly-Gly-Gly, wherein six of the Gly residues have a common molecular mass that is different from a common molecular mass of the remaining six of the Gly residues.
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67. A method comprising:
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labeling a protein or a peptide in a sample with a reporter signal peptide comprising the amino acid sequence Gly-Gly-Gly-Gly-Gly-Gly-Asp-Pro-Gly-Gly-Gly-Gly-Gly-Gly, wherein six of the Gly residues have a common molecular mass that is different from a common molecular mass of the remaining six of the Gly residues;
separating the labeled protein or peptide or fragments thereof from molecules having a different mass-to-charge ratio in a mass spectrometer;
fragmenting the reporter signal peptide by collision induced dissociation in an ion trap mass spectrometer; and
detecting fragmented reporter signal peptide. - View Dependent Claims (68, 69, 70, 71, 72, 73, 74, 75)
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- 94. A reporter signal peptide comprising a single Asn-Pro amino acid sequence, wherein the reporter signal peptide can be fragmented across the Asn-Pro peptide bond by chemical cleavage.
- 97. A reporter signal peptide comprising a single Glu-Pro amino acid sequence, wherein the reporter signal peptide can be fragmented across the Glu-Pro peptide bond by collision-induced dissociation in an ion trap mass spectrometer.
Specification