Proteinaceous pharmaceuticals and uses thereof
First Claim
1. A non-naturally occurring cysteine (C)-containing scaffold exhibiting a binding specificity towards a target molecule, comprising a polypeptide having two disulfide bonds formed by pairing intra-scaffold cysteines according to a pattern selected from the group consisting of C1-2 3-4, C1-3, 2-4, and C1-4, 2-3, wherein the two numerical numbers linked by a hyphen indicate which two cysteines counting from N-terminus of the polypeptide are paired to form a disulfide bond.
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Abstract
The present invention provides cysteine-containing scaffolds and/or proteins, expression vectors, host cell and display systems harboring and/or expressing such cysteine-containing products. The present invention also provides methods of designing libraries of such products, methods of screening such libraries to yield entities exhibiting binding specificities towards a taraget molecule. Further provided by the invention are pharmaceutical compositions comprising the cysteine-containing products of the present invention.
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Citations
20 Claims
- 1. A non-naturally occurring cysteine (C)-containing scaffold exhibiting a binding specificity towards a target molecule, comprising a polypeptide having two disulfide bonds formed by pairing intra-scaffold cysteines according to a pattern selected from the group consisting of C1-2 3-4, C1-3, 2-4, and C1-4, 2-3, wherein the two numerical numbers linked by a hyphen indicate which two cysteines counting from N-terminus of the polypeptide are paired to form a disulfide bond.
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2. A non-naturally occurring cysteine (C)-containing scaffold exhibiting a binding specificity towards a target molecule, comprising a polypeptide having three disulfide bonds formed by pairing intra-scaffold cysteines according to a pattern selected from the group consisting of C1-2, 3-4, 5-6, C1-2, 3-5, 4-6, C1-2, 3-6, 4-5, C1-2, 3-6, 5-6, C1-3, 2-5, 4-6, C1-3, 2-6, 4-5, C1-4, 2-3, 5-6, C1-4, 2-6, 3-5, C1-5, 2-3, 4-6, C1-5, 2-4, 3-6, C1-5, 2-6, 3-4, C1-6, 2-3, 4-5, and C1-6, 2-5, 3-4, wherein the two numerical numbers linked by a hyphen indicate which two cysteines counting from N-terminus of the polypeptide are paired to form a disulfide bond.
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3. A non-naturally occurring cysteine (C)-containing scaffold exhibiting a binding specificity towards a target molecule, comprising a polypeptide having at least four disulfide bonds formed by pairing intra-scaffold cysteines according to a pattern selected from the following:
Specification