Solid Support Having Ligand Immobilized Thereon By Using Photocleavable Linker
First Claim
1. A solid support for the analysis of a specific interaction between a ligand and a target molecule, wherein the ligand is immobilized via a linker cleavable by photoirradiation.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides a method of analyzing the interaction between a ligand and a target molecule, which uses a solid support wherein the ligand is immobilized via a linker cleavable by photoirradiation, particularly a method of searching and purifying a target molecule. By intervening a linker specifically cleavable by photoirradiation between the ligand molecule and the solid support, release and elution of the target molecule from the solid support without using a ligand or salt at a high concentration can be enabled, and suppression of release and elution of a nonspecific protein can be enabled.
-
Citations
18 Claims
- 1. A solid support for the analysis of a specific interaction between a ligand and a target molecule, wherein the ligand is immobilized via a linker cleavable by photoirradiation.
-
7. A compound represented by the following formula (I′
- ) or (II′
);
- ) or (II′
-
8. A linker used for immobilizing a ligand on a solid support, which is represented by the following formula (I′
- ) or (II′
);
- View Dependent Claims (9, 10)
- ) or (II′
- 11. A method of searching a target molecule, which comprises (1) a step of immobilizing a ligand on a solid support via a linker cleavable by photoirradiation, (2) a step of mixing a solid support with the ligand immobilized thereon, which is obtained in the above-mentioned (1), and a sample wherein a target molecule of the ligand is or is not contained, (3) a step of cleaving a ligand from the solid support by irradiation of light, and (4) a step of confirming binding of the target molecule to the ligand.
- 15. A method of purifying a target molecule, which comprises (1) a step of immobilizing a ligand on a solid support via a linker cleavable by photoirradiation, (2) a step of mixing a solid support with the ligand immobilized thereon, which is obtained in the above-mentioned (1), and a sample wherein a target molecule of the ligand is contained, (3) a step of cleaving a ligand from the solid support by irradiation of light, and (4) a step of recove
Specification