Biosensor membrane and methods related thereto

• US 20070215465A1
• Filed: 03/15/2006
• Published: 09/20/2007
• Est. Priority Date: 03/15/2006
• Status: Active Grant

• Current Assignee: Honeywell International Inc.
• Original Assignee: Honeywell International Inc.
• Inventors: Gu, Yuandong
• Granted Patent: US 8,679,308 B2
• Time in Patent Office: Days
• US Class Current: 204/403.10
• CPC Class Codes: C12Q 1/002 Electrode membranes

Abstract

Embodiments of the present invention relate to a sensor membrane. The sensor membrane comprises one or more conversion layers wherein the one or more conversion layers are capable of converting a non-charged analyte into a charged species, one or more polymeric layers wherein the one or more polymeric layers act as matrices to host the one or more conversion layers and wherein the one or more conversion layers and one or more polymeric layers create oppositely charged regions within the membrane.

Claims

1. A sensor membrane, comprising:

one or more conversion layers, wherein the one or more conversion layers are capable of converting a non-charged analyte into a charged species;

one or more polymeric layers, wherein the one or more polymeric layers act as matrices to host the one or more conversion layers;

and wherein the one or more conversion layers and one or more polymeric layers create oppositely charged regions within the membrane.

2. The sensor membrane of claim 1, wherein the total charge of the combination of one or more conversion layers, the one or more polymeric layers and mobile ions attracted to the layers in solution is neutral.

3. The sensor membrane of claim 1, wherein the conversion layer comprises a conversion species.

4. The sensor membrane of claim 3, wherein the conversion species is an enzyme.

5. The sensor membrane of claim 1, wherein the one or more conversion layers and the one or more polymeric layers comprise a polyelectrolyte.

6. The sensor membrane of claim 5, wherein the polyelectrolyte comprises poly(sodium styrene sulfonate) (PSS), poly(acrylic acid) (PAA), polylysine, polycyclic aromatic hydrocarbon (PAH) or combinations thereof.

7. The sensor of claim 1, wherein the one or more polymeric layers have a pKa value lower than the aqueous environment and lower than the operating pH of the sensor.

8. The sensor of claim 7, wherein the one or more polymeric layers have a pKa value at least two pH units lower than the aqueous environment and lower than the operating pH of the sensor.

9. A sensor, comprising:

a sensor surface,

a membrane, in contact with the sensor surface, the membrane comprising;

one or more conversion layers, wherein the one or more conversion layers are capable of converting a non-charged analyte into a charged species;

one or more polymeric layers, wherein the one or more polymeric layers act as matrices to host the one or more conversion layers;

and wherein the one or more conversion layers and one or more polymeric layers create oppositely charged regions within the membrane; and

a transducer.

10. The sensor membrane of claim 9, wherein the one or more conversion layers and the one or more polymeric layers comprise a polyelectrolyte.

11. The sensor membrane of claim 10, wherein the polyelectrolyte comprises poly(sodium styrene sulfonate) (PSS), poly(acrylic acid) (PAA), polylysine, polycyclic aromatic hydrocarbon (PAH) or combinations thereof.

12. The sensor of claim 9, wherein the one or more polymeric layers have a pKa value lower than the aqueous environment and lower than the operating pH of the sensor.

13. The sensor of claim 12, wherein the one or more polymeric layers have a pKa value at least two pH units lower than the aqueous environment and lower than the pH of the sensor.

14. A method of detecting an analyte in a fluid, the method comprising:

contacting an analyte and buffering species with a layered membrane, sufficient to create an analyte in a buffer selective environment;

converting the analyte, sufficient to produce a charged analyte; and

detecting the conversion of the analyte, sufficient to provide analyte concentration measurements.

16. A method of making a sensor membrane, comprising:

contacting a sensor surface with a first charged polyelectrolyte;

contacting the sensor surface with a second charged polyelectrolyte, wherein the second charged polyelectrolyte is oppositely charged in relation to the first polyelectrolyte, and

wherein the layered membrane produced is capable of converting a non-charged analyte into a charged species.

15. The method of claim 16, wherein detecting the conversion of the analyte comprises detection by calorimetric, amperometric, potentiometric, optical, piezo-electric means or combinations thereof.

17. The method of claim 16, wherein the first charged polyelectrolyte or the second charged polyelectrolyte comprise a conversion layer.

18. The method of claim 16, wherein the layered membrane has a thickness of about 0.001 microns to about 1000 microns.

19. The method of claim 16, wherein the layered membrane has a thickness of about 0.01 microns to about 500 microns.

20. A method of making a sensor membrane, comprising:

contacting a sensor surface with a first charged polyelectrolyte;

contacting the sensor surface with a neutral conversion layer;

contacting the sensor surface with a second charged polyelectrolyte, wherein the second charged polyelectrolyte is oppositely charged in relation to the first polyelectrolyte, and

wherein the layered membrane produced is capable of converting a non-charged analyte into a charged species.