Device And Method For Separating, Mixing And Concentrating Magnetic Particles With A Fluid And Use Thereof In Purification Methods
First Claim
1. A process for manipulating magnetic particles that are suspended in a fluid, the magnetic particles being able to bind an entity of interest, the fluid being contained in a reaction vessel constituted by a large upper compartment with a funnel shape, an elongate lower compartment with a substantially constant cross-section and a closed base, the process comprising:
- a) subjecting the magnetic particles to two magnetic fields applied simultaneously to separate all said magnetic particles present in at least the upper compartment of the vessel from the fluid, b) transferring the separated magnetic particles from the upper compartment to the elongated lower compartment, c) removing the fluid from the vessel, d) adding a washing liquid to the lower compartment, e) subjecting the rest of the fluid to at least two magnetic fields applied successively with different and changing directions to wash all the magnetic particles present in the lower compartment, and f) concentrating said magnetic particles in said lower compartment.
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Abstract
The present invention is related to a process for manipulating magnetic particles that are suspended in a fluid, possibly containing a biological entity of interest, the magnetic particles being able to bind the entity of interest, the fluid being contained in a reaction vessel constituted by a large upper compartment with a funnel shape, an elongate lower compartment with a substantially constant cross-section and a closed base. The process consists of: a) subjecting the magnetic particles to two magnetic fields applied simultaneously to separate all said magnetic particles present in at least the upper compartment of the vessel from the fluids b) transferring the separated magnetic particles from the upper compartment to the elongated lower compartment, c) removing the fluid from the vessel, d) adding a washing liquid to the lower compartment), e) subjecting the rest of the fluid to at least two magnetic fields applied successively with different and changing directions to wash all the magnetic particles present in the lower compartment, and concentrating said magnetic particles in said lower compartment The according to the present invention are especially useful in methods for the extraction of nucleic acid to enable them for further processing.
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Citations
34 Claims
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1. A process for manipulating magnetic particles that are suspended in a fluid, the magnetic particles being able to bind an entity of interest, the fluid being contained in a reaction vessel constituted by a large upper compartment with a funnel shape, an elongate lower compartment with a substantially constant cross-section and a closed base, the process comprising:
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a) subjecting the magnetic particles to two magnetic fields applied simultaneously to separate all said magnetic particles present in at least the upper compartment of the vessel from the fluid, b) transferring the separated magnetic particles from the upper compartment to the elongated lower compartment, c) removing the fluid from the vessel, d) adding a washing liquid to the lower compartment, e) subjecting the rest of the fluid to at least two magnetic fields applied successively with different and changing directions to wash all the magnetic particles present in the lower compartment, and f) concentrating said magnetic particles in said lower compartment. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. Device for extracting possible molecules of interest from a fluid to which is added a suspension of magnetic or (super) paramagnetic particles contained in at least one reaction vessel and capable to bind the molecules of interest comprising:
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at least one separating station to capture the magnetic particles present in a large upper compartment of each reaction vessel, at least one washing station to mix said magnetic particles present in a medium compartment of each reaction vessel, at least one concentrating station to mix said magnetic particles present in a bottom compartment of each reaction vessel, and at least one pipetting device to dispense and/or to remove part or all of the fluid, the washing liquid and output buffer used in an extraction process. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 31, 32, 33, 34)
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- 26. Set of reaction vessels constituted by at least two vessels, said vessels being arranged symmetrically along one line.
Specification