SAMPLE CONTAINER AND SYSTEM FOR A HANDHELD SPECTROMETER AND METHOD FOR USING THEREFOR

• US 20070216898A1
• Filed: 08/25/2006
• Published: 09/20/2007
• Est. Priority Date: 07/18/2003
• Status: Active Grant

• Current Assignee: Chemimage Corporation
• Original Assignee: Chemimage Corporation
• Inventors: Gardner, Charles
• Granted Patent: US 7,286,222 B2
• Time in Patent Office: Days
• US Class Current: 356/301
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/0205 Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
• CPC Class Codes: G01J 3/0218 using optical fibers
• CPC Class Codes: G01J 3/0224 using polarising or depolarising elements
• CPC Class Codes: G01J 3/0256 Compact construction
• CPC Class Codes: G01J 3/027 Control of working procedures of a spectrometer; Failure detection; Bandwidth calculation
• CPC Class Codes: G01J 3/0272 Handheld
• CPC Class Codes: G01J 3/0283 using a charging unit
• CPC Class Codes: G01J 3/0291 Housings; Spectrometer accessories; Spatial arrangement of elements, e.g. folded path arrangements
• CPC Class Codes: G01J 3/44 Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
• CPC Class Codes: G01N 2021/036 transformable, modifiable
• CPC Class Codes: G01N 2021/0389 Windows
• CPC Class Codes: G01N 2021/651 Cuvettes therefore
• CPC Class Codes: G01N 2021/656 Raman microprobe
• CPC Class Codes: G01N 21/0303 Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment G01N21/031 - G01N21/15 take precedence
• CPC Class Codes: G01N 21/31 Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry G01N21/72 takes precedence
• CPC Class Codes: G01N 21/65 Raman scattering

Abstract

A container for holding a sample and a system and method for a handheld spectrometer using the container is disclosed. In one embodiment, the container includes a vial with an optical window at the base of the vial. A sample may be placed in the vial. A hollow plunger may be slidably inserted into the vial which seals the gap between the plunger and the walls of the vial. The plunger includes a filter element. When the plunger is inserted into the vial, the sample is forced against the optical window and the filter element vents liquid and/or gas that is in the vial into the hollow plunger. A portable or handheld system for detecting, for example, biothreat agents makes use of the container in order to determine a spectrum of the sample. The optical window of the container is preferably substantially transparent to photons illuminating the sample and to photons produced due to the interaction of the illuminating photons and the sample.

Claims

1. A device for holding a sample, comprising:

an elongated hollow container open at a distal end and closed off at a proximal end by an optical window;

a hollow plunger configured to fit snugly into said hollow container via said open distal end and to slide within said hollow container so as to confine said sample in a first volume bounded by said hollow container, a bottom of the plunger, and said optical window;

said plunger comprising a filter attached to said bottom of the plunger to thereby allow a gas or liquid contained within said hollow container with said sample to be substantially transferred to a second volume partially bounded by said hollow plunger as said plunger slides within said hollow container; and

said optical window adapted to allow a first plurality of photons to pass therethrough into said first volume and to allow a second plurality of photons to pass therethrough out of said first volume.

2. The device of claim 1 wherein the elongated hollow container is substantially cylindrical.

3. The device of claim 1 wherein said optical window is substantially flat.

4. The device of claim 1 wherein said optical window comprises fused silica, plastic, quartz, glass, or a combination thereof.

5. The device of claim 1 wherein said first plurality of photons have a wavelength approximately equal to 532 nanometers.

6. The device of claim 1 wherein said first plurality of photons have wavelengths between 522 and 542 nanometers.

7. The device of claim 1 wherein said first plurality of photons have a wavelength in a range of wavelengths consisting of white light, near infrared light, infrared light, and ultraviolet light.

8. The device of claim 1 wherein said second plurality of photons comprise photons scattered by said sample.

9. The device of claim 8 wherein said scattered photons are Raman scattered photons.

10. The device of claim 1 wherein said second plurality of photons comprise photons emitted by said sample.

11. The device of claim 1 wherein said second plurality of photons comprise photons reflected by said sample.

12. The device of claim 1 wherein said sample is selected from the group consisting of:

biothreat agents, bacterial spores, live cells, virus, toxins, protozoan, protozoan cyst, and combinations thereof.

13. A system for obtaining a spectrum of a sample, comprising:

a photon source for providing a first plurality of photons;

a device for holding said sample, said device comprising;

an elongated hollow container open at a distal end and closed off at a proximal end by an optical window;

a hollow plunger configured to fit snugly into said hollow container via said open distal end and to slide within said hollow container so as to confine said sample in a first volume bounded by said hollow container, said plunger, and said optical window;

said plunger comprising a filter attached to said bottom of the plunger to thereby allow a gas or liquid contained within said hollow container with said sample to be substantially transferred to a second volume partially bounded by said hollow plunger as said plunger slides within said hollow container; and

said optical window adapted to allow said first plurality of photons to pass therethrough into said first volume and to allow a second plurality of photons to pass therethrough out of said first volume;

a first optical lens for collecting said second plurality of photons;

a filter for blocking a portion of said first plurality of photons present in an optical path with said second plurality of photons;

a second optical lens for directing said second photons into a fiber optic array; and

said fiber optic array for directing said second photons to a photon detector to thereby obtain a spectrum of said sample.

14. The system of claim 13 further comprising a beam expander for providing said second plurality of photons.

15. The system of claim 13 further comprising a dichroic mirror for filtering said first and second plurality of photons.

16. The system of claim 13 wherein said fiber optic array is a fiber array spectral translator.

17. The system of claim 13 wherein optical fibers comprising the fiber optic array are arranged in a two-dimensional array at a first end and a one-dimensional array at a second end of the fiber optic array.

18. The system of claim 17 wherein said first end accepts said second plurality of photons from said second optical lens and said second end directs said second plurality of photons to said photon detector.

19. The system of claim 13 wherein said photon detector is a charge-coupled device.

20. The system of claim 13 wherein said photon detector is a spectrometer.

21. The system of claim 13 further comprising a microprocessor for analyzing an output signal from said photon detector.

22. The system of claim 13 further comprising a memory device for storing a spectrum of a biothreat agent.

23. The system of claim 22 further comprising a microprocessor for comparing an output signal from said photon detector with the stored spectrum in said memory device.

24. The system of claim 23 further comprising a display unit for displaying information based on said comparison.

25. The system of claim 24 further comprising a device for accepting input from a user.

26. The system of claim 13 wherein the elongated hollow container is substantially cylindrical.

27. The device of claim 13 wherein said optical window is substantially flat.

28. The device of claim 13 wherein said optical window comprises fused silica, plastic, quartz, glass, or a combination thereof.

29. The device of claim 13 wherein said first plurality of photons have a wavelength approximately equal to 532 nanometers.

30. The device of claim 13 wherein said first plurality of photons have wavelengths between 522 and 542 nanometers.

31. The device of claim 13 wherein said first plurality of photons have a wavelength in a range of wavelengths consisting of white light, near infrared light, infrared light, and ultraviolet light.

32. The device of claim 13 wherein said second plurality of photons comprise photons scattered by said sample.

33. The device of claim 32 wherein said scattered photons are Raman scattered photons.

34. The device of claim 13 wherein said second plurality of photons comprise photons emitted by said sample.

35. The device of claim 13 wherein said second plurality of photons comprise photons reflected by said sample.

36. The device of claim 13 wherein said sample is selected from the group consisting of:

biothreat agents, bacterial spores, live cells, virus, toxins, protozoan, protozoan cyst, and combinations thereof.

37. The system of claim 13 wherein the system is portable.

38. The system of claim 37 wherein the system is hand-held.

39. A system for obtaining a spectrum of a sample, comprising:

a photon source for providing a first plurality of photons;

a beam expander for providing a second plurality of photons;

a dichroic mirror for filtering said first and second plurality of photons;

a device for holding said sample, said device comprising;

an elongated hollow container open at a distal end and closed off at a proximal end by an optical window;

a hollow plunger configured to fit snugly into said hollow container via said open distal end and to slide within said hollow container so as to confine said sample in a first volume bounded by said hollow container, said plunger, and said optical window;

said plunger comprising a filter attached to said bottom of the plunger to thereby allow a gas or liquid contained within said hollow container with said sample to be substantially transferred to a second volume partially bounded by said hollow plunger as said plunger slides within said hollow container; and

said optical window adapted to allow said first plurality of photons to pass therethrough into said first volume and to allow said second plurality of photons to pass therethrough out of said first volume;

a first optical lens for collecting said second plurality of photons;

a filter for blocking a portion of said first plurality of photons present in an optical path with said second plurality of photons;

a second optical lens for directing said second photons into a fiber optic array;

said fiber optic array for directing said second photons to a photon detector to thereby obtain a spectrum of said sample, wherein optical fibers comprising the fiber optic array are arranged in a two-dimensional array at a first end and a one-dimensional array at a second end of the fiber optic array, and wherein said first end accepts said second plurality of photons from said second optical lens and said second end directs said second plurality of photons to said photon detector;

a memory device for storing a spectrum of a biothreat agent;

a microprocessor for comparing an output signal from said photon detector with the stored spectrum in said memory device;

a display unit for displaying information based on said comparison; and

a device for accepting input from a user.

40. A method for obtaining a spectrum of a sample, comprising:

(a) illuminating said sample with a first plurality of photons;

(b) holding said sample using a device comprising;

(i) an elongated hollow container open at a distal end and closed off at a proximal end by an optical window;

(ii) a hollow plunger configured to fit snugly into said hollow container via said open distal end and to slide within said hollow container so as to confine said sample in a first volume bounded by said hollow container, said plunger, and said optical window;

(iii) said plunger comprising a filter attached to said bottom of the plunger to thereby allow a gas or liquid contained within said hollow container with said sample to be substantially transferred to a second volume partially bounded by said hollow plunger as said plunger slides within said hollow container; and

(iv) said optical window adapted to allow said first plurality of photons to pass therethrough into said first volume and to allow a second plurality of photons to pass therethrough out of said first volume;

(c) collecting said second plurality of photons;

(d) blocking a portion of said first plurality of photons present in an optical path with said second plurality of photons; and

(e) directing said second plurality of photons to a photon detector to thereby obtain a spectrum of said sample.

41. The method of claim 40 further comprising the steps of:

(f) storing a predetermined spectrum of a biothreat agent in a memory device;

(g) comparing an output signal from said photon detector with the stored spectrum; and

(h) displaying information based on said comparison.

42. The method of claim 40 wherein said first plurality of photons have a wavelength approximately equal to 532 nanometers.

43. The method of claim 40 wherein said first plurality of photons have a wavelength in a range of wavelengths consisting of white light, near infrared light, infrared light, and ultraviolet light.

44. The method of claim 40 wherein said second plurality of photons comprise photons scattered by said sample.

45. The method of claim 44 wherein said scattered photons are Raman scattered photons.

46. The method of claim 40 wherein said second plurality of photons comprise photons emitted by said sample.

47. The method of claim 40 wherein said second plurality of photons comprise photons reflected by said sample.

48. The method of claim 40 wherein said sample is selected from the group consisting of:

biothreat agents, bacterial spores, live cells, virus, toxins, protozoan, protozoan cyst, and combinations thereof.

49. A device comprising:

an elongated hollow container open at a distal end and closed off at a proximal end by an optical window;

a plunger configured to fit into said hollow container via said open distal end and to slide within said hollow container so as to confine a sample in a volume bounded by said hollow container, a bottom of the plunger, and said optical window, and also to allow a gas or liquid contained within said hollow container to be substantially transferred out of said hollow container when said plunger slides into said hollow container; and

said optical window adapted to allow a first plurality of photons to pass therethrough into said volume and to allow a second plurality of photons to pass therethrough out of said volume.

50. The device of claim 49 wherein said optical window is substantially flat.

51. The device of claim 49 wherein said optical window comprises fused silica, plastic, quartz, glass, or a combination thereof.

52. The device of claim 49 wherein said first plurality of photons have a wavelength in a range of wavelengths consisting of white light, near infrared light, infrared light, and ultraviolet light.

53. The device of claim 49 wherein said sample is selected from the group consisting of:

biothreat agents, bacterial spores, live cells, virus, toxins, protozoan, protozoan cyst, and combinations thereof.