Diagnostic System For Otolaryngologic Pathogens And Use Thereof
First Claim
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1. A method of detecting presence of an otolaryngologic pathogen in a biological sample:
- providing a sensor device comprising (i) a substrate having two or more nucleic acid probes respectively confined to two or more distinct locations thereon, and (ii) a detector that detects the binding of target nucleic acids of a biological sample to the two or more nucleic acid probes, wherein a target nucleic acid is specific to one or more otolaryngologic pathogens;
exposing the biological sample, or a portion thereof, to the sensor device under conditions effective to allow hybridization between the two or more nucleic acid probes and a target nucleic acid to occur; and
detecting with the detector whether any target nucleic acid hybridizes to the two or more nucleic acid probes, wherein hybridization indicates presence of the otolaryngologic pathogen in the biological sample and presence of more than one otolaryngologic pathogen can be detected simultaneously.
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Abstract
The present invention relates to a method of detecting the presence of an otolaryngologic pathogen in a biological sample, and a sensor device, sensor chip, and nucleic acid probes useful for detecting otolaryngologic pathogens.
100 Citations
56 Claims
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1. A method of detecting presence of an otolaryngologic pathogen in a biological sample:
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providing a sensor device comprising (i) a substrate having two or more nucleic acid probes respectively confined to two or more distinct locations thereon, and (ii) a detector that detects the binding of target nucleic acids of a biological sample to the two or more nucleic acid probes, wherein a target nucleic acid is specific to one or more otolaryngologic pathogens;
exposing the biological sample, or a portion thereof, to the sensor device under conditions effective to allow hybridization between the two or more nucleic acid probes and a target nucleic acid to occur; and
detecting with the detector whether any target nucleic acid hybridizes to the two or more nucleic acid probes, wherein hybridization indicates presence of the otolaryngologic pathogen in the biological sample and presence of more than one otolaryngologic pathogen can be detected simultaneously. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 35)
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24. A sensor device comprising:
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a substrate having two or more nucleic acid probes respectively confined to two or more distinct locations thereon and a detector that detects the hybridization of target nucleic acids to the two or more nucleic acid probes upon exposure to a biological sample, wherein a target nucleic acid is specific to one or more otolaryngologic pathogens and hybridization indicates presence of the otolaryngologic pathogen in the biological sample, the detector being capable of simultaneously detecting presence of more than one otolaryngologic pathogen in the biological sample. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 36, 37, 38)
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- 39. A sensor chip comprising a substrate having two or more nucleic acid probes respectively confined to two or more distinct locations thereon, the nucleic acid probes hybridizing to a target nucleic acid of an otolaryngologic pathogen under suitable hybridization conditions, wherein the two or more probes are selected to hybridize, collectively, to target nucleic acids of two or more otolaryngologic pathogens.
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54. A nucleic acid probe comprising a nucleic acid sequence selected from the group of:
SEQ ID NO;
1, SEQ ID NO;
2, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
8, SEQ ID NO;
9, SEQ ID NO;
10, SEQ ID NO;
11, SEQ ID NO;
12, SEQ ID NO;
13, SEQ ID NO;
14, SEQ ID NO;
15, SEQ ID NO;
16, SEQ ID NO;
17, SEQ ID NO;
18, SEQ ID NO;
19, SEQ ID NO;
20, SEQ ID NO;
21, SEQ ID NO;
22, and complements thereof; and
combinations thereof.
Specification