COMPOSITIONS FOR USE IN IDENTIFICATION OF BACTERIA
First Claim
Patent Images
1. An oligonucleotide primer pair comprising a forward primer and a reverse primer, each comprising between 13 and 35 linked nucleotides in length, configured to generate an amplicon that is between 45 and 200 linked nucleotides in length, said forward primer configured to hybridize with at least 70% complementarity to a first portion of a region of Genbank gi number:
- 57634611, and said reverse primer configured to hybridize with at least 70% complementarity to a second portion of said region of Genbank gi number;
57634611, wherein said region of Genbank gi number;
57634611 begins with the 5′
end of SEQ ID NO.;
174, and extends to the 5′
end of SEQ ID NO.;
899.
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Abstract
The present invention provides compositions, kits and methods for rapid identification and quantification of bacteria by molecular mass and base composition analysis.
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Citations
29 Claims
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1. An oligonucleotide primer pair comprising a forward primer and a reverse primer, each comprising between 13 and 35 linked nucleotides in length, configured to generate an amplicon that is between 45 and 200 linked nucleotides in length, said forward primer configured to hybridize with at least 70% complementarity to a first portion of a region of Genbank gi number:
- 57634611, and said reverse primer configured to hybridize with at least 70% complementarity to a second portion of said region of Genbank gi number;
57634611, wherein said region of Genbank gi number;
57634611 begins with the 5′
end of SEQ ID NO.;
174, and extends to the 5′
end of SEQ ID NO.;
899. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
- 57634611, and said reverse primer configured to hybridize with at least 70% complementarity to a second portion of said region of Genbank gi number;
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17. A kit for identifying a Staphylococcus aureus bioagent comprising:
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i) a first oligonucleotide primer pair comprising a forward primer and a reverse primer, each comprising between 13 and 35 linked nucleotides in length, configured to generate an amplicon that is between 45 and 200 linked nucleotides in length, said forward primer configured to hybridize with at least 70% complementarity to a first portion of a region of Genbank gi number;
57634611, and said reverse primer configured to hybridize with at least 70% complementarity to a second portion of said region of Genbank gi number;
57634611, wherein said region of Genbank gi number;
57634611 begins with the 5′
end of SEQ ID NO;
174 and extends to the 5′
end of SEQ ID NO;
899; and
ii) at least one additional primer pair, wherein the primers of each of said at least one additional primer pair are configured to hybridize to conserved sequence regions within a Staphylococcus aureus gene selected from the group consisting of;
mecA, mecRI, ermA, ermC, pvluk, tufB and mupR. - View Dependent Claims (18, 19)
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20. A method for identifying a Staphylococcus aureus bioagent in a sample comprising:
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a) amplifying a nucleic acid from said sample using an oligonucleotide primer pair comprising a forward primer and a reverse primer, each comprising between 13 and 35 linked nucleotides in length, said forward primer configured to hybridize with at least 70% complementarity to a first portion of a region of Genbank gi number;
57634611, and said reverse primer configured to hybridize with at least 70% complementarity to a second portion of said region of Genbank gi number;
57634611, wherein said region of Genbank gi number;
57634611 begins with the 5′
end of SEQ ID NO.;
174, and extends to the 5′
end of SEQ ID NO.;
899;
wherein said amplifying generates at least one amplification product that comprises between 45 and 200 linked nucleotides; and
b) determining the molecular mass of said at least one amplification product by mass spectrometry. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29)
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Specification