Compositions comprising conditioned cell culture media and uses thereof

• US 20070218549A1
• Filed: 01/04/2007
• Published: 09/20/2007
• Est. Priority Date: 06/07/2001
• Status: Abandoned Application

• Current Assignee: Skinmedica Incorporated (Abbvie Incorporated)
• Original Assignee: Skinmedica Incorporated (Abbvie Incorporated)
• Inventors: Mansbridge, Jonathan
• Application Number:
  US11/619,630
• Publication Number:
  US 20070218549A1
• Time in Patent Office: Days
• US Class Current: 435/325
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 2800/522 Antioxidants; Radical scavengers
• CPC Class Codes: A61K 2800/86 Products or compounds obtained by genetic engineering
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 38/1825 Fibroblast growth factor [FGF]
• CPC Class Codes: A61K 38/1866 Vascular endothelial growth factor [VEGF]
• CPC Class Codes: A61K 38/44 Oxidoreductases (1)
• CPC Class Codes: A61K 38/446 Superoxide dismutase (1.15)
• CPC Class Codes: A61K 8/447 containing sulfur
• CPC Class Codes: A61K 8/64 Proteins; Peptides; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/678 Tocopherol, i.e. vitamin E
• CPC Class Codes: A61P 9/06 Antiarrhythmics
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin
• CPC Class Codes: C07K 14/52 Cytokines; Lymphokines; Interferons
• CPC Class Codes: C07K 2319/01 containing a localisation/targetting motif
• CPC Class Codes: C07K 2319/10 containing a tag for extracellular membrane crossing, e.g. TAT or VP22
• CPC Class Codes: C07K 2319/75 containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
• CPC Class Codes: C12N 15/62 DNA sequences coding for fusion proteins
• CPC Class Codes: C12N 2500/90 Serum-free medium, which may still contain naturally-sourced components
• CPC Class Codes: C12N 2502/1323 Adult fibroblasts
• CPC Class Codes: C12N 2510/02 : Cells for production

Abstract

The invention relates to compositions comprising cell culture medium conditioned by cells grown in three-dimensional culture. The cells used to condition the medium may be genetically modified to alter the concentration of growth factors and antioxidants in the medium. The conditioned cell medium (conditioned medium) may be used for at least one of cosmetic applications, cosmeceutical applications, and pharmaceutical applications, among other things. The invention also relates to proteins comprising a heterologous sequence that enhances cell penetration. The invention also relates to cells comprising DNA encoding such proteins. Methods for preparing the inventive compounds are also provided.

Claims

1-62. -62. (canceled)

63. A growth factor comprising a heterologous peptide sequence that enhances cell penetration.

64. The growth factor of claim 63, wherein the growth factor comprises at least one of insulin, insulin-like growth factor (IGF), nerve growth factor, VEGF, keratinocyte growth factor (KGF), fibroblast growth factor (FGF), platelet-derived growth factor (PDGF), hepatocyte growth factor (HGF), transforming growth factor alpha (TGFα

), transforming growth factor beta (TGFβ

), epidermal growth factor (EGF), granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), interleukin-6 (IL-6), and interleukin-8 (IL-8); and

wherein the heterologous peptide sequence comprises at least one of the following sequences;

SEQ ID NO;

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7, SEQ ID NO;

8, SEQ ID NO;

9, SEQ ID NO;

10, SEQ ID NO;

11, SEQ ID NO;

12, SEQ ID NO;

13, SEQ ID NO;

14, SEQ ID NO;

15, SEQ ID NO;

16, SEQ ID NO;

17, SEQ ID NO;

18, and SEQ ID NO;

19.

65. An antioxidant comprising a heterologous peptide sequence that enhances cell penetration.

66. The antioxidant of claim 65, wherein the antioxidant comprises at least one of cysteine, cystine, ascorbic acid, glutathione, glutathione disulfide, glutathione peroxidase, glutathione reductase, glutathione disulfide, superoxide dismutase, catalase, vitamin E, ascorbic acid, ubiquinol 9, and ubiquinone 9;

and wherein the heterologous peptide sequence comprises at least one of the following sequences;

SEQ ID NO;

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7, SEQ ID NO;

8, SEQ ID NO;

9, SEQ ID NO;

10, SEQ ID NO;

11, SEQ ID NO;

12, SEQ ID NO;

13, SEQ ID NO;

14, SEQ ID NO;

15, SEQ ID NO;

16, SEQ ID NO;

17, SEQ ID NO;

18, and SEQ ID NO;

19.

67. An extracellular matrix component comprising a heterologous peptide sequence that enhances cell penetration.

68. The extracellular matrix component of claim 67, wherein the extracellular matrix component comprises at least one of:

at least one glycoprotein, at least one proteoglycan, and at least one glycosaminoglycan; and

wherein the heterologous peptide sequence comprises at least one of the following sequences;

SEQ ID NO;

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7, SEQ ID NO;

8, SEQ ID NO;

9, SEQ ID NO;

10, SEQ ID NO;

11, SEQ ID NO;

12, SEQ ID NO;

13, SEQ ID NO;

14, SEQ ID NO;

15, SEQ ID NO;

16, SEQ ID NO;

17, SEQ ID NO;

18, and SEQ ID NO;

19.

69. A cell comprising DNA encoding a growth factor comprising a heterologous peptide sequence that enhances cell penetration.

70. A cell according to claim 69, wherein the growth factor comprises at least one of insulin, insulin-like growth factor (IGF), nerve growth factor, VEGF, keratinocyte growth factor (KGF), fibroblast growth factor (FGF), platelet-derived growth factor (PDGF), hepatocyte growth factor (HGF), transforming growth factor alpha (TGFa), transforming growth factor beta (TGF, epidermal growth factor (EGF), granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), interleukin-6 (IL-6), and interleukin-8 (IL-8);

and wherein the heterologous peptide sequence comprises at least one of the following sequences;

SEQ ID NO;

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7, SEQ ID NO;

8, SEQ ID NO;

9, SEQ ID NO;

10, SEQ ID NO;

11, SEQ ID NO;

12, SEQ ID NO;

13, SEQ ID NO;

14, SEQ ID NO;

15, SEQ ID NO;

16, SEQ ID NO;

17, SEQ ID NO;

18, and SEQ ID NO;

19.

71. A cell comprising DNA encoding an antioxidant comprising a heterologous peptide sequence that enhances cell penetration.

72. A cell according to claim 71, wherein the antioxidant comprises at least one of cysteine, cystine, ascorbic acid, glutathione, glutathione disulfide, glutathione peroxidase, glutathione reductase, glutathione disulfide, superoxide dismutase, catalase, vitamin E, ascorbic acid, ubiquinol 9, and ubiquinone 9;

and wherein the heterologous peptide sequence comprises at least one of the following sequences;

SEQ ID NO;

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7, SEQ ID NO;

8, SEQ ID NO;

9, SEQ ID NO;

10, SEQ ID NO;

11, SEQ ID NO;

12, SEQ ID NO;

13, SEQ ID NO;

14, SEQ ID NO;

15, SEQ ID NO;

16, SEQ ID NO;

17, SEQ ID NO;

18, and SEQ ID NO;

19.

73. A cell comprising DNA encoding an extracellular matrix component comprising a heterologous peptide sequence that enhances cell penetration.

74. A cell according to claim 73, wherein the extracellular matrix component comprises at least one of:

at least one glycoprotein, at least one proteoglycan, and at least one glycosaminoglycan; and

wherein the heterologous peptide sequence comprises at least one of the following sequences;

SEQ ID NO;

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7, SEQ ID NO;

8, SEQ ID NO;

9, SEQ ID NO;

1O, SEQ ID NO;

11, SEQ ID NO;

12, SEQ ID NO;

13, SEQ ID NO;

14, SEQ ID NO;

15, SEQ ID NO;

16, SEQ ID NO;

17, SEQ ID NO;

18, and SEQ ID NO;

19.