Sample analyzer

• US 20070222973A1
• Filed: 03/16/2007
• Published: 09/27/2007
• Est. Priority Date: 03/16/2006
• Status: Active Grant

• Current Assignee: Sysmex Corporation
• Original Assignee: Sysmex Corporation
• Inventors: Yamamoto, Norimasa, Matsuo, Naohiko, Hoshiko, Susumu, Kobayashi, Katsushi
• Granted Patent: US 7,760,340 B2
• Time in Patent Office: Days
• US Class Current: 356/39
• CPC Class Codes: G01J 2003/123 Indexed discrete filters
• CPC Class Codes: G01N 2021/3148 using three or more wavelengths
• CPC Class Codes: G01N 2021/3155 Measuring in two spectral ranges, e.g. UV and visible
• CPC Class Codes: G01N 2021/3174 Filter wheel
• CPC Class Codes: G01N 2021/825 Agglutination
• CPC Class Codes: G01N 2035/00356 Holding samples at elevated temperature (incubation)
• CPC Class Codes: G01N 2035/0455 Coaxial carousels
• CPC Class Codes: G01N 21/0332 with temperature control control of temperature G05D23/00; cryostats F17C3/08
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/272 for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/314 with comparison of measurements at specific and non-specific wavelengths dual wavelength spectrometry G01J3/427
• CPC Class Codes: G01N 21/82 producing a precipitate or turbidity
• CPC Class Codes: G01N 2201/0618 Halogene sources
• CPC Class Codes: G01N 2201/0826 Fibre array at source, distributing
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80
• CPC Class Codes: G01N 35/00603 Reinspection of samples

Abstract

A sample analyzer comprising: a measuring part for measuring optical information of a sample at first wavelength, second wavelength, and third wavelength, first light of the first wavelength and second light of the second wavelength being absorbed by a second substance but substantially not absorbed by a first substance, and third light of the third wavelength being absorbed by the first substance; and an obtaining means for obtaining content of the first substance in the sample, and content of the second substance in the sample, influence by the second substance being excluded from the content of the first substance, based on the optical information at the first wavelength, second wavelength, and third wavelength measured by the measuring part.

Claims

1. A sample analyzer comprising:

a measuring part for measuring optical information of a sample at first wavelength, second wavelength, and third wavelength, first light of the first wavelength and second light of the second wavelength being absorbed by a second substance but substantially not absorbed by a first substance, and third light of the third wavelength being absorbed by the first substance; and

an obtaining means for obtaining content of the first substance in the sample, and content of the second substance in the sample, influence by the second substance being excluded from the content of the first substance, based on the optical information at the first wavelength, second wavelength, and third wavelength measured by the measuring part.

2. The sample analyzer according to claim 1, wherein

the obtaining means comprises:

an estimating means for estimating the influence by the second substance on the optical information at the third wavelength based on the optical information at the first wavelength and second wavelength; and

a correcting means for correcting the optical information at the third wavelength based on the estimated influence by the second substance at the third wavelength.

3. The sample analyzer according to claim 2, further comprising:

a first obtaining means for obtaining information relating to presence of the second substance in the sample based on at least one of the optical information at the first wavelength and the second wavelength;

a second obtaining means for obtaining information relating to presence of the first substance in the sample based on the optical information at the third wavelength corrected by the correcting means; and

an output means for outputting at least one of the information obtained by the first obtaining means and the information obtained by the second obtaining means.

4. The sample analyzer according to claim 1, wherein

the first light, the second light, and the third light are substantially not absorbed by a third substance;

the measuring part is configured so as to further measure optical information of the sample at fourth wavelength, fourth light of the fourth wavelength being absorbed by the third substance;

the obtaining means is configured so as to obtain content of the third substance in the sample, influence by the first substance and the second substance being excluded from the content of the third substance, based on the optical information at the first wavelength, second wavelength, third wavelength, and fourth wavelength measured by the measuring part.

5. The sample analyzer according to claim 4, wherein

the obtaining means comprises:

a first estimating means for estimating the influence by the second substance on the optical information at the third wavelength based on the optical information at the first wavelength and second wavelength;

a first correcting means for correcting the optical information at the third wavelength based on the estimated influence by the second substance at the third wavelength;

a second estimating means for estimating the influence by the second substance on the optical information at the fourth wavelength based on the optical information at the first wavelength and second wavelength;

a third estimating means for estimating the influence by the first substance on the optical information at the fourth wavelength based on the optical information at the third wavelength corrected by the first correcting means; and

a second correcting means for correcting the optical information at the fourth wavelength based on the influence by the first substance at the fourth wavelength estimated by the third estimating means and the influence by the second substance at the fourth wavelength estimated by the second estimating means.

6. The sample analyzer according to claim 5, further comprising:

a first obtaining means for obtaining information relating to presence of the second substance in the sample based on at least one optical information at the first wavelength and the second wavelength;

a second obtaining means for obtaining information relating to presence of the first substance in the sample based on the optical information at the third wavelength corrected by the first correcting means;

a third obtaining means for obtaining information relating to presence of the third substance in the sample based on the optical information at the fourth wavelength corrected by the second correcting means; and

an output means for outputting at least one of information obtained by the first obtaining means, second obtaining means, and third obtaining means.

7. The sample analyzer according to claim 1, further comprising:

a preparing means for preparing a measurement sample by mixing a reagent with the sample;

wherein the measuring part is configured so as to measure optical information of the measurement sample,

the sample analyzer further comprises;

an analyzing means for analyzing the sample based on the optical information of the measurement sample;

an output means for outputting an analysis result of the measurement sample obtained by the analyzing means, and

wherein the first substance and second substance are interference substances that interfere the optical measurement relating to a target substance in the measurement sample.

8. The sample analyzer according to claim 4, further comprising:

a preparing means for preparing a measurement sample by mixing a reagent with the sample;

wherein the measuring part is configured so as to measure optical information of the measurement sample,

the sample analyzer further comprises;

an analyzing means for analyzing the sample based on the optical information of the measurement sample;

an output means for outputting an analysis result of the measurement sample obtained by the analyzing means, and

wherein the first substance, second substance, and third substance are interference substances that interfere the optical measurement relating to a target substance in the measurement sample.

9. The sample analyzer according to claim 1, wherein

the measuring part comprises:

a light source that emits the first light of the first wavelength, the second light of the second wavelength, and the third light of the third wavelength; and

a light receiver that receives the first light, the second light, and the third light.

10. The sample analyzer according to claim 2, wherein

the estimating means is configured so as to estimate optical information corresponding to the second substance at the third wavelength based on optical information at the first wavelength and second wavelength;

and

the correcting means is configured so as to correct optical information at the third wavelength by eliminating optical information corresponding to the second substance at the third wavelength estimated by the estimating means from the optical information of the sample at the third wavelength.

11. The sample analyzer according to claim 10, wherein

the optical information is absorbance;

and

the estimating means is configured so as to estimate absorbance corresponding to the second substance at the third wavelength from a relation derived from the absorbance at the first wavelength and absorbance at the second wavelength.

12. The sample analyzer according to claim 2, wherein

the estimating means is configured so as to estimate optical information corresponding to the second substance at the third wavelength based on optical information at the first wavelength and second wavelength;

and

the correcting means is configured so as to correct optical information at the third wavelength by eliminating optical information corresponding to the second substance at the third wavelength estimated by the estimating means from the optical information of the sample at the third wavelength.

13. The sample analyzer according to claim 5, wherein

the first estimating means is configured so as to estimate optical information corresponding to the second substance at the third wavelength based on optical information at the first wavelength and second wavelength;

the first correcting means is configured so as to correct optical information at the third wavelength by eliminating optical information corresponding to the second substance at the third wavelength estimated by the estimating means from the optical information of the sample at the third wavelength;

the second estimating means is configured so as to estimate optical information corresponding to the second substance at the fourth wavelength based on optical information at a first wavelength and second wavelength;

the third estimating means is configured so as to estimate optical information corresponding to the first substance at the fourth wavelength based on the optical information at the third wavelength corrected by the first correcting means; and

the second correcting means is configured so as to correct optical information at the fourth wavelength by eliminating optical information corresponding to the first substance at the fourth wavelength estimated by the third estimating means, and optical information corresponding to the second substance at the fourth wavelength estimated by the second estimating means from the optical information of the sample at the fourth wavelength.

14. The sample analyzer according to claim 1, wherein

the first wavelength and second wavelength are equal to or greater than 590 nm and equal to or less than 900 nm.

15. The sample analyzer according to claim 1, wherein

the third wavelength is equal to or greater than 500 nm and less than 590 nm.

16. The sample analyzer according to claim 4, wherein

the fourth wavelength is equal to or greater than 300 nm and less than 500 nm.

17. A sample analyzer comprising:

a sample preparing part for preparing a measurement sample by mixing a reagent for measuring coagulation time with a blood sample;

a light emitting part for emitting light to the prepared measurement sample;

a light receiving part for obtaining optical information at a plurality of time points by receiving light of a plurality of wavelengths over time from the measurement sample irradiated by the light;

a first measuring means for measuring content of an interference substance in the measurement sample that interferes optical measurement of the measurement sample based on optical information obtained by the light receiving part at a time point before the measurement sample indicates coagulation reaction; and

a second measuring means for measuring coagulation time of the measurement sample based on the optical information obtained by the light receiving part.

18. The sample analyzer according to claim 17, wherein

the first measuring means is configured so as to respectively measure content of a plurality of interference substances based on optical information at a plurality of wavelengths obtained by the light receiving part.

19. The sample analyzer according to claim 17, wherein

the light receiving part is configured so as to obtain at least optical information at a first wavelength, second wavelength, and third wavelength;

the first measuring means is configured so as to measure at least one of bilirubin, hemoglobin, and chyle in the blood sample based on at least one optical information at the first wavelength, the second wavelength, and the third wavelength obtained by the light receiving part.

20. The sample analyzer according to claim 19, wherein

first light of the first wavelength is absorbed by chyle, and is substantially not absorbed by bilirubin and hemoglobin;

second light of the second wavelength is absorbed by chyle and hemoglobin, and is substantially not absorbed by bilirubin;

third light of the third wavelength is absorbed by chyle, hemoglobin and bilirubin;

the first measuring means comprises;

a first estimating means for estimating influence by chyle on the optical information at the second wavelength and third wavelength based on the optical information at the first wavelength; and

a second estimating means for estimating influence by hemoglobin on the optical information at the third wavelength, based on the optical information at the second wavelength and the influence by chyle at the second wavelength;

wherein

contents of bilirubin, hemoglobin, and chyle in the blood sample are measured based on the optical information at the first wavelength, second wavelength, and third wavelength;

the influence by chyle on the optical information at the second wavelength and third wavelength estimated by the first estimating means; and

the influence by hemoglobin on the optical information at the third wavelength estimated by the second estimating means.

21. The sample analyzer according to claim 20, wherein

the light receiving part is configured so as to obtain optical information at a fourth wavelength, fourth light of the fourth wavelength being absorbed by chyle but being substantially not absorbed by bilirubin and hemoglobin;

and

the first estimating means is configured so as to estimate the influence by chyle on the optical information at the second wavelength and third wavelength based on the optical information at the first wavelength and fourth wavelength.

22. The sample analyzer according to claim 17, wherein

the light receiving part is configured so as to obtain optical information at a first wavelength, first light of the first wavelength being absorbed by chyle but being substantially not absorbed by bilirubin and hemoglobin, and at a second wavelength, second light of the second wavelength being absorbed by chyle and hemoglobin but being substantially not absorbed by bilirubin, and a third wavelength, third light of the third wavelength being absorbed by bilirubin, hemoglobin, and chyle;

and

the first measuring means comprises;

a first obtaining means for obtaining influence by chyle on optical information based on optical information at the first wavelength obtained by the light receiving part;

a second obtaining means for obtaining influence by hemoglobin on optical information based on optical information at the second wavelength obtained by the light receiving part, and the influence by chyle on optical information obtained by the first obtaining means;

a third obtaining means for obtaining influence by bilirubin on optical information based on optical information at the third wavelength obtained by the light receiving part, the influence by hemoglobin on optical information obtained by the second obtaining means, and the influence by chyle on optical information obtained by the first obtaining means;

the sample analyzer further comprises;

an output part for outputting the influence by chyle on optical information obtained by the first obtaining means, the influence by hemoglobin on optical information obtained by the second obtaining means, and the influence by bilirubin on optical information obtained by the third obtaining means.

23. The sample analyzer according to claim 22, wherein

the first obtaining means obtains the influence by chyle on optical information based on optical information corresponding to the reagent obtained by irradiating the reagent with light, in addition to the optical information at the first wavelength.

24. The sample analyzer according to claim 17, wherein

the light emitting part is configured so as to emit light of a plurality of mutually different wavelengths at time divisions.

25. The sample analyzer according to claim 17, further comprising:

a second light receiving part for obtaining optical information by receiving light from the blood sample before mixed with a measuring reagent;

wherein

the first measuring means is configured so as to measure content of interference substances in the blood sample that optically interferes the optical measurement of the blood sample based on optical information obtained by the second light receiving part.

26. The sample analyzer according to claim 25, further comprising:

a deciding means for deciding whether or not to obtain optical information by the light receiving part based on the optical information obtained by the second light receiving part.

27. The sample analyzer according to claim 25, further comprising:

a selecting means for selecting any one of the content of the interference substance in the measurement sample based on optical information obtained by the second light receiving means, and the content of the interference substance in the blood sample measured based on optical information obtained by the light receiving part.

28. A sample analyzer comprising:

a sample preparing part for preparing a measurement sample by mixing a reagent for measuring coagulation time with a blood sample;

a light emitting part for emitting light to the prepared measurement sample;

a light receiving part for obtaining optical information at a plurality of time points by receiving light of a plurality of wavelengths over time from the measurement sample irradiated by the light;

a specifying means for specifying an interference substance in the measurement sample that interferes optical measurement of the measurement sample, based on optical information at a time point before the measurement sample indicates coagulation reaction; and

a measuring means for measuring coagulation time of the measurement sample based on the optical information obtained by the light receiving part.