COMPOSITIONS FOR USE IN IDENTIFICATION OF BACTERIA
First Claim
1. An oligonucleotide primer pair comprising a forward and a reverse primer, each comprising between 13 and 35 linked nucleotides in length, designed to generate an amplicon that is between about 45 and about 200 linked nucleotides in length, wherein said forward primer comprises at least 80% complementarity to a first region within nucleotides 1-286 of a reference sequence, said reference sequence being a sequence extraction of coordinates 830671-831072 of Genbank gi number 21281729, and wherein said reverse primer comprises at least 80% complementarity to a second region within nucleotides 1-286 of said reference sequence.
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Abstract
The present invention provides compositions, kits and methods for rapid identification and quantification of bacteria by molecular mass and base composition analysis.
121 Citations
27 Claims
- 1. An oligonucleotide primer pair comprising a forward and a reverse primer, each comprising between 13 and 35 linked nucleotides in length, designed to generate an amplicon that is between about 45 and about 200 linked nucleotides in length, wherein said forward primer comprises at least 80% complementarity to a first region within nucleotides 1-286 of a reference sequence, said reference sequence being a sequence extraction of coordinates 830671-831072 of Genbank gi number 21281729, and wherein said reverse primer comprises at least 80% complementarity to a second region within nucleotides 1-286 of said reference sequence.
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21. A method for identifying, determining one or more characteristics of, or detecting a Staphylococcus aureus bioagent in a sample comprising:
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a) amplifying a nucleic acid from said sample using an oligonucleotide primer pair targeted to a Staphylococcus aureus tpi gene comprising a forward and a reverse primer, each being between 13 and 35 linked nucleotides in length, wherein said forward primer comprises at least 70% complementarity to a first region within nucleotides 1-286 of a reference sequence, said reference sequence being a sequence extraction of coordinates 830671-831072 of Genbank gi number 21281729, and wherein said reverse primer comprises at least 70% complementarity to a second region within nucleotides 1-286 of said reference sequence, wherein said amplifying generates at least one amplification product that comprises between about 45 and about 200 linked nucleotides; and
b) determining the molecular mass of said at least one amplification product by mass spectrometry. - View Dependent Claims (22, 23, 24, 25, 26, 27)
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Specification