Oxidative protection of lipid layer biosensors
First Claim
1. A method for preparing a lipid layer on a solid support, said method comprising:
- providing a solid support;
contacting said solid support with a solution, wherein said solution comprises a lipid layer forming compound and at least one lipid-soluble antioxidant compound at a concentration of about 1 μ
M to about 10 mM.
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Accused Products
Abstract
The present invention provides methods and composition related to lipid layers with increased stability to oxidative degradation. In one embodiment, the invention provides lipid layers comprising antioxidant inclusion molecules that permit biosensors with increased performance and functional lifetime due to increased oxidative protection. In addition to these lipid-layer compositions, the present invention provides methods for further protection of lipid layers from oxidation. These methods include application of solutions and coatings comprising barrier compounds (e.g., trehalose), storage under anaerobic conditions (e.g., with inert gases), storage with oxidant scavengers (e.g., dessicants and catalysts), and packaging with barrier materials (e.g., mylar, polyethylene) that prevent oxidant exposure.
26 Citations
33 Claims
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1. A method for preparing a lipid layer on a solid support, said method comprising:
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providing a solid support;
contacting said solid support with a solution, wherein said solution comprises a lipid layer forming compound and at least one lipid-soluble antioxidant compound at a concentration of about 1 μ
M to about 10 mM. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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- 11. A lipid layer on a solid support with increased resistance to oxidation comprising a close-packed layer of an amphiphilic molecule, wherein the layer includes at least one lipid soluble antioxidant compound at a concentration of about 0.1 ppm to about 5000 ppm.
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19. A method for preparing a lipid layer on a solid support for storage, said method comprising:
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providing a solid support comprising a lipid layer;
contacting the lipid layer with a barrier compound solution, wherein the barrier compound is selected from the group consisting of;
trehalose, polyvinylalcohol, cationic polymers, starch, polystyrene sulfonate, polyethylene glycol, and polyethylene oxide. - View Dependent Claims (20, 21, 22, 23, 24, 25)
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26. An ion channel sensor comprising:
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a solid support with a conducting surface;
a lipid bilayer with first and second layers each comprising closely packed amphiphilic molecules, wherein the first layer is attached to the conducting surface of the solid support and comprises a first ionophore, and wherein the second layer comprises a second ionophore, and at least one lipid soluble antioxidant compound at a concentration of about 0.1 ppm to about 5000 ppm;
a plurality of recognition molecules covalently attached to the second ionophores, wherein the recognition molecules are capable of binding to an analyte.
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27. An ion channel sensor product comprising:
an ion channel sensor sealed in an oxidant barrier material container under anaerobic conditions, wherein the ion channel sensor comprises;
a solid support with a conducting surface;
a lipid bilayer with first and second layers each comprising closely packed amphiphilic molecules, wherein the first layer is attached to the conducting surface of the solid support and comprises a first ionophore, and wherein the second layer comprises a second ionophore, and at least one lipid soluble antioxidant compound at a concentration of about 0.1 ppm to about 5000 ppm;
a plurality of recognition molecules covalently attached to the second ionophores, wherein the recognition molecules are capable of binding to an analyte. - View Dependent Claims (28, 29, 30, 31, 32, 33)
Specification