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Chemoselective Fluorgenic Molecular Linkers and Methods for Their Preparation and Use

  • US 20070224695A1
  • Filed: 04/15/2005
  • Published: 09/27/2007
  • Est. Priority Date: 04/16/2004
  • Status: Active Grant
First Claim
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1. A method of forming a linkage complex having a fluorescent signal, the method comprising:

  • contacting a first linkage compound having the structure A-(Y)y

    N3, or A-(Y)y

    X;

    with a second linkage compound having the structure E-(Z)z-C≡

    CRE, or E-(Z)z-C≡

    N;

    to form a linkage complex having a fluorescent signal that is different than the fluorescent signal of the first linkage compound and the fluorescent signal of the second linkage compound;

    where;

    at least one of A and E is a fluorophore;

    X is halo, and when the first linkage compound is A-(Y)y

    X, the first linkage compound is contacted with the second linkage compound in the presence of an azide salt of lithium, sodium, or potassium;

    RE is selected from any chemically possible substituent group;

    y and z are each independently an integer from 0 to 20; and

    Y and Z are each independently a branched or unbranched alkyl, alkenyl, or alkynyl, which can be substituted or unsubstituted.

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