Monte Carlo based model of fluorescence in turbid media and methods and systems for using same to determine intrinsic fluorescence of turbid media
First Claim
1. A method for modeling fluorescence of a turbid medium and for using the model to determine intrinsic fluorescence in the turbid medium, the method comprising:
- illuminating a turbid medium of interest with an electromagnetic radiation source using a probe of a particular geometry and detecting measured fluorescence for the turbid medium using the probe;
running at least one set of Monte Carlo simulations to determine an escape energy probability map and an absorbed energy density map for the turbid medium; and
determining, in a manner that accounts for the geometry of the probe, and using the measured fluorescence, the absorbed energy density map, and the escape energy probability map, an indication of intrinsic fluorescence of the turbid medium.
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Abstract
The subject matter described herein includes a method for modeling fluorescence in turbid media and methods and systems for using the model to determine intrinsic fluorescence of turbid media. According to one aspect, a method for modeling fluorescence of a turbid medium and for using the model to determine intrinsic fluorescence in the turbid medium is provided. The method includes illuminating a turbid medium of interest with an electromagnetic radiation source using a probe of a particular geometry and detecting measured fluorescence for the turbid medium using the probe. At least one set of Monte Carlo simulations is run to determine an escape energy probability map and an absorbed energy density map for the turbid medium. An indication of the intrinsic fluorescence of the turbid medium is determined using the escape probability density map and the absorbed energy density map in a manner that accounts for the geometry of the probe.
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Citations
20 Claims
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1. A method for modeling fluorescence of a turbid medium and for using the model to determine intrinsic fluorescence in the turbid medium, the method comprising:
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illuminating a turbid medium of interest with an electromagnetic radiation source using a probe of a particular geometry and detecting measured fluorescence for the turbid medium using the probe;
running at least one set of Monte Carlo simulations to determine an escape energy probability map and an absorbed energy density map for the turbid medium; and
determining, in a manner that accounts for the geometry of the probe, and using the measured fluorescence, the absorbed energy density map, and the escape energy probability map, an indication of intrinsic fluorescence of the turbid medium. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A system for modeling fluorescence of a turbid medium and for using the model to determine intrinsic fluorescence in the turbid medium, the system comprising:
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a probe for illuminating a turbid medium of interest with electromagnetic radiation generated by an electromagnetic radiation source, the probe having a particular geometry and the probe detecting measured fluorescence for the turbid medium; and
a processor for running at least one set of Monte Carlo simulations to determine an escape energy probability map and an absorbed energy density map for the turbid medium, for determining, using the measured fluorescence, the absorbed energy density map, and the escape energy probability map, wavelength-dependent extinction coefficients and emission spectra for a known fluorophore, and accounting for the geometry of the probe, an indication of intrinsic fluorescence of the turbid medium. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification