METHODS, ARRANGEMENTS AND SYSTEMS FOR OBTAINING INFORMATION ASSOCIATED WITH AN ANATOMICAL SAMPLE USING OPTICAL MICROSCOPY

• US 20070233056A1
• Filed: 02/08/2007
• Published: 10/04/2007
• Est. Priority Date: 02/08/2006
• Status: Active Grant

• Current Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Original Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Inventors: Yun, Seok-Hyun
• Granted Patent: US 9,777,053 B2
• Time in Patent Office: Days
• US Class Current: 606/32
• CPC Class Codes: A61B 3/102 for optical coherence tomography [OCT]
• CPC Class Codes: A61B 3/117 for examining the anterior chamber or the anterior chamber angle, e.g. gonioscopes
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/0066 Optical coherence imaging
• CPC Class Codes: A61B 5/0073 by tomography, i.e. reconstruction of 3D images from 2D projections A61B5/0066 takes precedence
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence
• CPC Class Codes: A61B 5/0086 using infrared radiation
• CPC Class Codes: A61B 5/0095 by applying light and detecting acoustic waves, i.e. photoacoustic measurements
• CPC Class Codes: A61B 5/0097 by applying acoustic waves and detecting light, i.e. acoustooptic measurements
• CPC Class Codes: A61B 5/4088 Diagnosing of monitoring cognitive diseases, e.g. Alzheimer, prion diseases or dementia
• CPC Class Codes: A61B 5/412 Detecting or monitoring sepsis
• CPC Class Codes: A61B 5/413 Monitoring transplanted tissue or organ, e.g. for possible rejection reactions after a transplant
• CPC Class Codes: C07K 14/70503 Immunoglobulin superfamily
• CPC Class Codes: C07K 2319/30 Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
• CPC Class Codes: C07K 2319/32 fusions with soluble part of a cell surface receptor, "decoy receptors"
• CPC Class Codes: C07K 2319/70 containing domain for protein-protein interaction
• CPC Class Codes: G01B 9/02091 Tomographic interferometers, e.g. based on optical coherence
• CPC Class Codes: G01J 3/4412 Scattering spectrometry particle sizing by light scattering G01N15/0205; optical velocimetry of particles G01P5/20, G01P5/26
• CPC Class Codes: G01N 2021/1725 Modulation of properties by light, e.g. photoreflectance
• CPC Class Codes: G01N 2021/638 : Brillouin effect, e.g. stimulated Brillouin effect
• CPC Class Codes: G01N 21/1717 : with a modulation of one or more physical properties of the sample during the optical investigation, e.g. electro-reflectance
• CPC Class Codes: G01N 21/4795 : spatially resolved investigating of object in scattering medium in vivo A61B
• CPC Class Codes: G01N 21/636 : using an arrangement of pump beam and probe beam; using the measurement of optical non-linear properties; non-linear optics per se G02F1/35
• CPC Class Codes: G01N 2201/06 : Illumination; Optics
• CPC Class Codes: G01N 2201/0612 : Laser diodes
• CPC Class Codes: G01N 2800/042 : Disorders of carbohydrate metabolism, e.g. diabetes, glucose metabolism
• CPC Class Codes: G01N 2800/387 : Down syndrome; Trisomy 18; Trisomy 13
• CPC Class Codes: G01N 2800/7023 : (Hyper)proliferation
• CPC Class Codes: G01N 2800/7028 : Cancer
• CPC Class Codes: G02B 21/0052 : Optical details of the image generation

Abstract

Arrangements and methods are provided for obtaining information associated with an anatomical sample. For example, at least one first electro-magnetic radiation can be provided to the anatomical sample so as to generate at least one acoustic wave in the anatomical sample. At least one second electro-magnetic radiation can be produced based on the acoustic wave. At least one portion of at least one second electro-magnetic radiation can be provided so as to determine information associated with at least one portion of the anatomical sample. In addition, the information based on data associated with the second electro-magnetic radiation can be analyzed. The first electro-magnetic radiation may include at least one first magnitude and at least one first frequency. The second electro-magnetic radiation can include at least one second magnitude and at least one second frequency. The data may relate to a first difference between the first and second magnitudes and/or a second difference between the first and second frequencies. The second difference may be approximately between −100 GHz and 100 GHz, excluding zero.

Claims

1. An arrangement comprising:

at least one first arrangement configured to provide at least one first electro-magnetic radiation to an anatomical sample so as to generate at least one acoustic wave in the anatomical sample, wherein at least one second electro-magnetic radiation is produced based on the at least acoustic wave; and

at least one second arrangement configured to receive at least one portion of the at least one second electro-magnetic radiation so as to determine information associated with at least one portion of the anatomical sample.

2. The arrangement according to claim 1, wherein the anatomical sample is at least one of an organ, a tissue or cell.

3. The arrangement according to claim 1, further comprising at least one third arrangement configured to image the at least one portion of the anatomical sample based on data associated with the at least one second electro-magnetic radiation.

4. The arrangement according to claim 3, further comprising at least one fourth arrangement configured to translate the at least one electro-magnetic radiation across the anatomical sample.

5. The arrangement according to claim 4, wherein the at least one fourth arrangement includes at least one lens, and wherein the at least one lens focuses the at least one first electro-magnetic radiation and collects the at least one second electro-magnetic radiation.

6. The arrangement according to claim 3, wherein the at least one first electro-magnetic radiation includes at least one first magnitude and at least one first frequency, wherein the at least one second electro-magnetic radiation includes at least one second magnitude and at least one second frequency, and wherein the data relates to at least one of a first difference between the first and second magnitudes or a second difference between the first and second frequencies.

7. The arrangement according to claim 6, wherein the second difference is approximately between −

100 GHz and 100 GHz, excluding zero.

8. The arrangement according to claim 1, wherein the at least one second arrangement includes a spectral filter which facilitates a determination of a spectrum of the at least one second electro-magnetic radiation.

9. The arrangement according to claim 4, wherein the at least one fourth arrangement is at least one of a catheter, an endoscope, a laparoscope, or an ophthalmic visualization device.

10. The arrangement according to claim 1, wherein the information is associated with a biomechanical property of the anatomical sample.

11. The arrangement according to claim 1, wherein the anatomical sample is a living subject.

12. The arrangement according to claim 1, wherein the at least one first electromagnetic radiation has a center wavelength which is approximately between 0.5-1.8 μ

m.

13. The arrangement according to claim 12, wherein the at least one first electromagnetic radiation has a line width which is smaller than approximately 100 MHz.

14. The arrangement according to claim 12, wherein the at least one first electromagnetic radiation has a form of a plurality of pulses, and wherein the duration of each of the pulses is longer than approximately 10 ns.

15. The arrangement according to claim 1, wherein the at least one first electro-magnetic radiation includes at least two first electro-magnetic radiations, wherein a first one of the at least two first electro-magnetic radiations has a first frequency, and a second one of the at least two first electro-magnetic radiations has a second frequency, and wherein a difference between the first and second frequencies is between approximately zero and 100 GHz.

16. The arrangement according to claim 1, wherein the at least one first electro-magnetic radiation includes at least one first magnitude and at least one first frequency, wherein the at least one second electro-magnetic radiation includes at least one second magnitude and at least one second frequency, wherein the at least one second arrangement is configured to receive at least one third electro-magnetic radiation which has at least one third magnitude and at least one third frequency, and wherein the second frequency is determined as a function of the first and third frequencies.

17. The arrangement according to claim 16, wherein the at least one third electro-magnetic radiation is forwarded from an electromagnetic radiation source.

18. An arrangement comprising:

at least one first arrangement configured to provide at least one first electro-magnetic radiation to an anatomical sample, wherein at least one second electro-magnetic radiation is provided from the anatomical sample;

at least one second arrangement configured to receive at least one portion of the at least one second electro-magnetic radiation so as to determine information associated with at least one portion of the anatomical sample; and

at least one third arrangement configured to analyze the information based on data associated with the at least one second electro-magnetic radiation,

wherein the at least one first electro-magnetic radiation includes at least one first magnitude and at least one first frequency, wherein the at least one second electro-magnetic radiation includes at least one second magnitude and at least one second frequency, and wherein the data relates to at least one of a first difference between the first and second magnitudes or a second difference between the first and second frequencies, and wherein the second difference is approximately between −

100 GHz and 100 GHz, excluding zero.

19. The arrangement according to claim 18, further comprising at least one fourth arrangement configured to image the at least one portion of the anatomical sample based on data associated with the at least one second electro-magnetic radiation.

20. The arrangement according to claim 19, further comprising at least one fifth arrangement configured to translate the at least one electro-magnetic radiation across the anatomical sample.

21. The arrangement according to claim 20, wherein the at least one fifth arrangement includes at least one lens, and wherein the at least one lens focuses the at least one first electro-magnetic radiation and collects the at least one second electro-magnetic radiation.

22. The arrangement according to claim 18, wherein the at least one second arrangement includes a spectral filter which facilitates a determination of a spectrum of the at least one second electro-magnetic radiation.

23. The arrangement according to claim 18, wherein the information is associated with a biomechanical property of the anatomical sample.

24. The arrangement according to claim 18, wherein the anatomical sample is a living subject.

25. The arrangement according to claim 18, wherein the at least one first electromagnetic radiation has a center wavelength which is approximately between 0.5-1.8 μ

m.

26. The arrangement according to claim 25, wherein the at least one first electromagnetic radiation has a line width which is smaller than approximately 100 MHz.

27. The arrangement according to claim 25, wherein the at least one first electromagnetic radiation has a form of a plurality of pulses, and wherein the duration of each of the pulses is longer than approximately 10 ns.

28. The arrangement according to claim 18, wherein the at least one first electro-magnetic radiation includes at least two first electro-magnetic radiations, wherein a first one of the at least two first electro-magnetic radiations has a first frequency, and a second one of the at least two first electro-magnetic radiations has a second frequency, and wherein a difference between the first and second frequencies is between approximately zero and 100 GHz.

29. The arrangement according to claim 18, wherein the at least one second arrangement is configured to receive at least one third electro-magnetic radiation which has at least one third magnitude and at least one third frequency, and wherein the second frequency is determined as a function of the first and third frequencies.

30. The arrangement according to claim 29, wherein the at least one third electro-magnetic radiation is forwarded from an electromagnetic radiation source.

31. A method for obtaining information associated with an anatomical sample, comprising:

providing at least one first electro-magnetic radiation to the anatomical sample so as to generate at least one acoustic wave in the anatomical sample, wherein at least one second electro-magnetic radiation is produced based on the at least acoustic wave; and

receiving at least one portion of at least one second electro-magnetic radiation so as to determine information associated with at least one portion of the anatomical sample.

32. A method for obtaining information associated with an anatomical sample, comprising:

providing at least one first electro-magnetic radiation to an anatomical sample, wherein at least one second electro-magnetic radiation is provided from the anatomical sample;

receiving at least one portion of the at least one second electro-magnetic radiation so as to determine information associated with at least one portion of the anatomical sample; and

analyzing the information based on data associated with the at least one second electro-magnetic radiation,

wherein the at least one first electro-magnetic radiation includes at least one first magnitude and at least one first frequency, wherein the at least one second electro-magnetic radiation includes at least one second magnitude and at least one second frequency, and wherein the data relates to at least one of a first difference between the first and second magnitudes or a second difference between the first and second frequencies, and wherein the second difference is approximately between −

100 GHz and 100 GHz, excluding zero.