Electrophoretic Devices, Instruments and Systems Including Same
First Claim
1. An electrophoretic device comprising:
- a separation chamber having a fluid inlet port and a fluid outlet port, with a flow path from the fluid inlet port to the fluid outlet port defining a fluid flow direction through the separation chamber;
an electrode chamber separated from the separation chamber by a porous, conductive membrane; and
electrodes positioned in the electrode chamber and operative when energized to generate an electric field gradient in the separation chamber, wherein the electrode chamber has a non-uniform configuration along at least a portion of the flow path of the separation chamber.
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Accused Products
Abstract
Devices, systems and methods are provided for processing fluid samples containing one or more analytes. In certain examples, an electrophoretic device comprises a separation chamber and an electrode chamber, each of which may be uniform or non-uniform. The electrode chamber includes two or more electrodes or may include an electrode array. Molecular sieve may be included in the separation chamber, and the molecular sieve is operative to shift the location at which a stationary focused band of a charged analyte forms under a given set of focusing process parameters. Such systems are especially suitable for linking up analytical instruments in a hyphenated fashion, particularly where the instruments have differing system parameters.
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Citations
86 Claims
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1. An electrophoretic device comprising:
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a separation chamber having a fluid inlet port and a fluid outlet port, with a flow path from the fluid inlet port to the fluid outlet port defining a fluid flow direction through the separation chamber;
an electrode chamber separated from the separation chamber by a porous, conductive membrane; and
electrodes positioned in the electrode chamber and operative when energized to generate an electric field gradient in the separation chamber, wherein the electrode chamber has a non-uniform configuration along at least a portion of the flow path of the separation chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A processing system comprising:
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a first sample treatment device having a fluid outlet port;
a second sample treatment device having a fluid inlet port and optionally having at least one operative fluidic requirement different from a corresponding operative fluidic requirement of the first sample treatment device; and
an electrophoretic device comprising a separation chamber having a fluid inlet port in fluid communication with the fluid outlet port of the first sample treatment device and a fluid outlet port in fluid communication with the fluid inlet port of the second sample treatment device, with a flow path from the fluid inlet port of the separation chamber to the fluid outlet port of the separation chamber defining a fluid flow direction through the separation chamber, and electrodes operative to generate an electric field gradient in the separation chamber. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56)
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57. A method for focusing a charged analyte comprising:
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providing a device for focusing a charged analyte comprising a separation chamber having a fluid inlet port and a fluid outlet port, with a flow path from the fluid inlet port to the fluid outlet port defining a fluid flow direction through the separation chamber, an electrode chamber separated from the separation chamber by a porous, conductive membrane, and electrodes positioned in the electrode chamber and operative when energized to generate an electric field gradient in the separation chamber, wherein the electrode chamber has a non-uniform configuration along at least a portion of the flow path of the separation chamber;
introducing a fluid sample comprising analyte into the separation chamber via the inlet port; and
energizing the electrodes to establish an electric field gradient in the separation chamber to focus at least a portion of the analyte at a location along the flow path. - View Dependent Claims (58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69)
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70. A method for focusing a charged analyte comprising:
providing a device for focusing a charged analyte comprising;
a separation chamber and a non-uniform electrode chamber separated from the separation chamber by a substantially planar, porous, conductive membrane, with electrodes positioned proximate the electrode chamber and operative to generate an electric field in the electrode channel;
introducing a first liquid comprising a plurality of charged analytes into the separation chamber; and
applying an electric field gradient to the plurality of charged analytes in the first liquid to focus the plurality of charged analytes in the electric field gradient into stationary focused bands of charged analytes, each charged analyte forming a stationary focused band.
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71. A method of processing a charged analyte comprising:
providing a processing system comprising;
a first sample treatment device having a fluid outlet port, a second sample treatment device having a fluid inlet port and optionally having at least one operative fluidic requirement different from a corresponding operative fluidic requirement of the first sample treatment device, and an electrophoretic device comprising a separation chamber having a fluid inlet port in fluid communication with the fluid outlet port of the first sample treatment device and a fluid outlet port in fluid communication with the fluid inlet port of the second sample treatment device, with a flow path from the fluid inlet port of the separation chamber to the fluid outlet port of the separation chamber defining a fluid flow direction through the separation chamber, and electrodes operative to generate an electric field gradient in the separation chamber;
passing a flow of fluid from the outlet port of the first sample treatment device to the fluid inlet port of the separation chamber;
focusing at least a portion of the analyte at a location in the separation chamber, comprising establishing an electric field gradient in the separation chamber by energizing the electrodes; and
topassing a flow of fluid comprising at least a portion of the analyte from the separation chamber to the inlet port of the second sample treatment device. - View Dependent Claims (72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86)
Specification