Closed flow-through microplate and methods for using and manufacturing same
First Claim
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1. A microplate, comprising:
- an upper plate including a top surface, a body and a bottom surface, where;
said top surface has located thereon a sealing substance which has one or more fluid delivery/removal sealing interfaces where each fluid delivery/removal sealing interface has one or more inlet ports and one or more outlet ports; and
said body has one or more fluid delivery/removal channels extending therethrough where each fluid delivery/removal channel has one or more inlet channels and one or more outlet channels which are respectively aligned with the one or more inlet ports and the one or more outlet ports located within the corresponding fluid delivery/removal sealing interface of said sealing substance; and
a lower plate including a top surface which is attached to said bottom surface of said upper plate such that one or more flow chambers are present there between, where each one of the flow chambers is in communication with a corresponding one of the fluid delivery/removal channels extending through said body of said upper plate.
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Abstract
A closed flow-through microplate is described herein that can be used to perform high-throughput kinetic flow-through assays to detect biomolecular interactions like material bindings, adsorptions etc. . . . that is helpful for example with testing new drugs. A method for manufacturing the closed flow-through microplate is also described herein.
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Citations
18 Claims
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1. A microplate, comprising:
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an upper plate including a top surface, a body and a bottom surface, where; said top surface has located thereon a sealing substance which has one or more fluid delivery/removal sealing interfaces where each fluid delivery/removal sealing interface has one or more inlet ports and one or more outlet ports; and said body has one or more fluid delivery/removal channels extending therethrough where each fluid delivery/removal channel has one or more inlet channels and one or more outlet channels which are respectively aligned with the one or more inlet ports and the one or more outlet ports located within the corresponding fluid delivery/removal sealing interface of said sealing substance; and a lower plate including a top surface which is attached to said bottom surface of said upper plate such that one or more flow chambers are present there between, where each one of the flow chambers is in communication with a corresponding one of the fluid delivery/removal channels extending through said body of said upper plate. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method for using a microplate, said method comprising the steps of:
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attaching a fluid delivery system to said microplate, where said fluid delivery system has one or more sets of fluid delivery/removal tips where each set of fluid delivery/removal tips has one or more fluid delivery tips and one or more fluid removal tips all of which are inserted into said microplate, where said microplate includes; an upper plate having a top surface, a body and a bottom surface, where; said top surface has located thereon a sealing substance which has one or more fluid delivery/removal sealing interfaces where each fluid delivery/removal sealing interface has one or more inlet ports and one or more outlet ports, where the inlet port(s) are size to receive and seal the fluid delivery tip(s) and the outlet port(s) are sized to receive and seal the fluid removal tip(s); and said body has one or more fluid delivery/removal channels extending therethrough where each fluid delivery/removal channel has one or more inlet channels and one or more outlet channels which are respectively aligned with the one or more inlet ports and the one or more outlet ports located within the corresponding fluid delivery/removal sealing interface; and a lower plate having a top surface which is attached to said bottom surface of said upper plate such that one or more flow chambers are present there between, where each one of the flow chambers is in communication with a corresponding one of the fluid delivery/removal channels extending through said body; inserting one or more fluids within each set of fluid delivery/removal tips and in particular the fluid delivery tip(s) such that the fluid(s) flow through the inlet channel(s) and the flow chamber(s) within said microplate; and removing the fluid(s) from each set of fluid delivery/removal tips and in particular the fluid removal tip(s) such that the fluid(s) are able to flow through the flow chamber(s) and out the outlet channel(s) within said microplate. - View Dependent Claims (8, 9, 10, 11, 12)
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13. A method of manufacturing a microplate, said method comprising the steps of:
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injection molding an upper plate which includes a top surface, a body and a bottom surface, where; said top surface has located thereon one or more depressions configured to receive one or more sealing substances; and said body has one or more fluid delivery/removal channels where each fluid delivery/removal channel has one or more inlet channels and one or more outlet channels extending there through and opening at said bottom surface; injection molding the sealing substance(s) into the depression(s) located within said top surface of said upper plate; attaching a top surface of a lower plate to said bottom surface of said upper plate such that one or more flow chambers are present there between, where each one of the flow chambers is in communication with one of the fluid delivery/removal channels extending through said body of said upper plate which in turn are in communication with one or more inlet ports and one or more outlet ports located within one or more fluid delivery/removal sealing interfaces of said sealing substance. - View Dependent Claims (14, 15, 16, 17, 18)
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Specification