Systems and methods of determining alleles and/or copy numbers
First Claim
Patent Images
1. A solid support, comprising:
- in a first region, a first nucleic acid; and
in second, third, fourth, and fifth regions, respectively, second, third, fourth, and fifth nucleic acids, each having a length that is shorter than the length of the first nucleic acid, each being immobilized relative to the solid support at a first end of each respective nucleic acid, each of the second, third, fourth, and fifth nucleic acids being substantially identical, wherein no two of the second, third, fourth, and fifth nucleic acids ends with identical terminal nucleotides.
2 Assignments
0 Petitions
Accused Products
Abstract
Various aspects of the invention include a solid support having a first region with a first nucleic acid, and second, third, fourth and fifth regions having, respectively, second, third, fourth, and fifth nucleic acids each having a length that is shorter than the length of the first nucleic acid. Certain aspects of the invention further include embodiments where the second, third, fourth, and fifth nucleic acids are substantially identical, and no two of the second, third, fourth, and fifth nucleic acids ends with identical terminal nucleotides. Some aspects of the invention further include methods of using the solid support, and kits that include the same.
65 Citations
20 Claims
-
1. A solid support, comprising:
-
in a first region, a first nucleic acid; and
in second, third, fourth, and fifth regions, respectively, second, third, fourth, and fifth nucleic acids, each having a length that is shorter than the length of the first nucleic acid, each being immobilized relative to the solid support at a first end of each respective nucleic acid, each of the second, third, fourth, and fifth nucleic acids being substantially identical, wherein no two of the second, third, fourth, and fifth nucleic acids ends with identical terminal nucleotides. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
-
-
12. A method, comprising acts of:
-
providing a first nucleic acid;
providing at least three types of substantially identical nucleic acid probes, each having a length that is shorter than the length of the first nucleic acid no two of the at least three types of substantially identical nucleic acid probes ending with identical terminal nucleotides;
hybridizing a target nucleic acid to at least some of the nucleic acid probes, the target nucleic acid having a length greater than the length of the nucleic acid probes;
substantially complementarily extending one of the probe nucleic acids along the target nucleic acid, without substantially extending the length of the other probe nucleic acids by subjecting the probe nucleic acids to primer extension reaction conditions;
evaluating binding of the target nucleic acid and the first nucleic acid; and
evaluating binding of the target acid with the nucleic acid probes. - View Dependent Claims (13, 14, 15, 16, 17, 18)
-
-
19. A solid support, comprising:
in first, second, third, fourth, and fifth regions, respectively, first, second, third, fourth, and fifth nucleic acids, each of the second, third, fourth, and fifth nucleic acids being substantially identical and having between 20 nucleotides and 50 nucleotides, inclusive, wherein the first nucleic acid comprises a portion substantially identical to the second, third, fourth, and fifth nucleic acids.
-
20. A kit, comprising:
-
a first nucleic acid; and
second, third, fourth, and fifth nucleic acids, each having a length that is shorter than the length of the first nucleic acid, each of the second, third, fourth, and fifth nucleic acid being substantially identical, wherein no two of the second, third, fourth, and fifth nucleic acids ends with identical terminal nucleotides.
-
Specification