Validation of comparative genomic hybridization
First Claim
Patent Images
1. A method for validating a CGH assay, the method comprising acts of:
- selecting a sequence within a target DNA based on the results of a CGH assay;
exposing the target DNA to an oligonucleotide probe comprising a sequence able to hybridize to a portion of the target DNA; and
determining association of the oligonucleotide probe with the target DNA, thereby validating the CGH assay.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention generally relates to techniques involving comparative genomic hybridization (CGH) and related techniques, including the validation of assay results. In one aspect, a region of interest of a genome or other target nucleic acid, identified using CGH or similar techniques, may be validated using a probe based on the CGH results. The oligonucleotides, in some embodiments, may bind the genome in some fashion (e.g., to the region of interest, and/or to other predetermined regions), and thus can be used for validation of CGH or other results.
60 Citations
20 Claims
-
1. A method for validating a CGH assay, the method comprising acts of:
-
selecting a sequence within a target DNA based on the results of a CGH assay;
exposing the target DNA to an oligonucleotide probe comprising a sequence able to hybridize to a portion of the target DNA; and
determining association of the oligonucleotide probe with the target DNA, thereby validating the CGH assay. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
-
-
13. A method for validating a genomic region of interest, the method comprising acts of:
-
selecting a genomic region of interest;
exposing the genomic region of interest to an oligonucleotide probe comprising a sequence able to hybridize to a portion of the genomic region of interest; and
determining association of the oligonucleotide probe with the genomic region of interest. - View Dependent Claims (14)
-
-
15. A composition, comprising:
a plurality of oligonucleotide probes, including a first oligonucleotide probe and a second oligonucleotide probe different from the first oligonucleotide probe, each of the first and second oligonucleotide probes comprising respective first and second sequences able to specifically hybridize to respective first and second portions of a genome, wherein the first portion and the second portion are in contact or are separated along the genome by a distance of no more than 1,000 bases. - View Dependent Claims (16)
-
17. A method, comprising:
synthesizing a first oligonucleotide probe and a second oligonucleotide probe different from the first oligonucleotide probe, each of the first and second oligonucleotide probes comprising respective first and second sequences able to specifically hybridize to respective first and second portions of a genome, wherein the first portion and the second portion are in contact or are separated along the genome by a distance of no more than 1,000 bases.
-
18. A kit, comprising:
-
a first oligonucleotide probe; and
a second oligonucleotide probe different from the first oligonucleotide probe, wherein each of the first and second oligonucleotide probes comprises respective first and second sequences able to specifically hybridize to respective first and second portions of a genome, wherein the first portion and the second portion are in contact or are separated along the genome by a distance of no more than 1,000 bases.
-
-
19. A method, comprising:
-
synthesizing a plurality of oligonucleotides on a substrate, including a first oligonucleotide probe and a second oligonucleotides different from the first oligonucleotide probe, each of the first and second oligonucleotides comprising respective first and second sequences able to specifically hybridize to respective first and second portions of a genome, wherein the first portion and the second portion are in contact or are separated along the genome by a distance of no more than 1,000 bases; and
cleaving at least some of the oligonucleotides from the substrate.
-
-
20. An article, comprising:
an array comprising a plurality of oligonucleotide probes, including a first oligonucleotide probe and a second oligonucleotide probe different from the first oligonucleotide probe, each of the first and second oligonucleotide probes comprising respective first and second sequences able to specifically hybridize to respective first and second portions of a genome, wherein the first portion and the second portion are in contact or are separated along the genome by a distance of no more than 1,000 bases.
Specification