Amperometric sensor for uric acid and method for the same
First Claim
1. A manufacturing method for an amperometric sensor for uric acid, comprising the steps of:
- (a) fixing a conducting wire on a carbon electrode with a silver paste and then baking the carbon electrode in an oven;
(b) packaging the portion of the sensor device containing the conducting wire with epoxy and then baking the sensor in an oven to manufacture a tri-polar carbon electrode;
(c) forming the tri-polar carbon electrode having a sensing window thereon;
(d) mixing polyacrylamide and a phosphate solution;
(e) forming a polyacrylamide solution by mixing the polyacrylamide used in (d) with ferrocenecarboxylic acid;
(f) diluting uricase with the polyacrylamide solution obtained in (e); and
(g) dripping a proper amount of the diluted uricase obtained in (f) on the sensing window, and distributing the diluted uricase uniformly thereon, and then air-drying the diluted uricase to fix it, and the amperometric sensor for uric acid is achieved;
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Abstract
An amperometric sensor for uric acid and a manufacturing method thereof are disclosed, in which polyacrylamide is used to fix catalase, uricase and ferrocenecarboxylic acid on a working electrode. In determining concentration of uric acid, hydrogen peroxide is produced when enzyme and uric acid react with each other and then a reduction current generated from enzyme on the electrode with an external voltage 200 mV applied is detected. In determining concentration of uric acid, a concentration range of 2.5-20 mg/dl is achieved and sensibility of the sensor in a linear portion is 5.17 uAcm−2(mg/dl)−1. In addition, reaction time required for the reaction between enzyme and uric acid is 5.17 uAcm−2(mg/dl)−1.
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Citations
11 Claims
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1. A manufacturing method for an amperometric sensor for uric acid, comprising the steps of:
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(a) fixing a conducting wire on a carbon electrode with a silver paste and then baking the carbon electrode in an oven;
(b) packaging the portion of the sensor device containing the conducting wire with epoxy and then baking the sensor in an oven to manufacture a tri-polar carbon electrode;
(c) forming the tri-polar carbon electrode having a sensing window thereon;
(d) mixing polyacrylamide and a phosphate solution;
(e) forming a polyacrylamide solution by mixing the polyacrylamide used in (d) with ferrocenecarboxylic acid;
(f) diluting uricase with the polyacrylamide solution obtained in (e); and
(g) dripping a proper amount of the diluted uricase obtained in (f) on the sensing window, and distributing the diluted uricase uniformly thereon, and then air-drying the diluted uricase to fix it, and the amperometric sensor for uric acid is achieved;
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification