Flow Paths Comprising One or Two Porous Beds
First Claim
1. A microfluidic device comprising a microchannel structure in which there are one, two or more flow paths all of which comprises a porous bed I that is common for all of the flow paths, which bed exposes an immobilized reactant R that is capable of interacting with a solute S that passes through the bed, wherein at least one of the flow paths comprises a second porous bed II that is placed upstream of porous bed I and is dummy with respect to interaction with solute S but capable of interacting with a substance DS that is present in a liquid aliquot together with solute S and is capable of disturbing the result of the interaction between solute S and said immobilized reactant R.
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Abstract
A microfluidic device that comprises a microchannel structure in which there are one, two or more flow paths (101;201a,b;301a,a′,b) all of which comprises a porous bed I (104,204,304) that is common for all of the flow paths and exposes an immobilized reactant R that is capable of interacting with a solute S that passes through the bed. The characteristics are that at least one of the flow paths comprises/comprise a second porous bed II (105,205,305) that is placed upstream of porous bed I (104,204,304) and is dummy with respect to interaction with solute S but capable of interacting with a substance DS that is present in a liquid aliquot together with solute S and is capable of disturbing the result of the interaction between solute S and said immobilized reactant R. There is also disclosed a method utilizing the device and variant of the device in which the immobilized R is replaced with a generic affinity ligand LI and/or porous bed II exposes a generic ligand LII that may be different from LI.
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Citations
17 Claims
- 1. A microfluidic device comprising a microchannel structure in which there are one, two or more flow paths all of which comprises a porous bed I that is common for all of the flow paths, which bed exposes an immobilized reactant R that is capable of interacting with a solute S that passes through the bed, wherein at least one of the flow paths comprises a second porous bed II that is placed upstream of porous bed I and is dummy with respect to interaction with solute S but capable of interacting with a substance DS that is present in a liquid aliquot together with solute S and is capable of disturbing the result of the interaction between solute S and said immobilized reactant R.
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11. A microfluidic process carried out in a flow path of a microchannel structure of a microfluidic device and comprising transporting a liquid aliquot containing a solute S through a porous bed I that is placed in said flow path and exhibits an immobilized reactant R that is capable of interacting with solute S during the transport, characterized in comprising the steps of
(i) providing said flow path in a form that comprises a porous bed II that is upstream of porous bed I and dummy with respect to interaction with solute S but capable of interacting with a disturbing substance DS, (ii) providing a liquid aliquot containing said solute S and said disturbing substance in said flow path in a position that is upstream of porous bed II, (iii) transporting the aliquot through porous bed II, and (iv) transporting subsequently solute S through porous bed I to allow for the interaction with reactant R.
- 12. A microfluidic device comprising a microchannel structure that comprises one, two or more flow paths each of which comprises a porous bed I that is common for all of said flow paths and at least one of which comprises a porous bed II which is upstream of porous bed I, wherein one or both of porous bed I and porous bed II in said at least one flow path comprises a solid phase material containing a generic ligand.
Specification