Cell and sub-cell methods for imaging and therapy
First Claim
1. A method of forming loaded cells, cell-derived vesicles or synthetic vesicles for facilitating in vivo imaging, targeting or biological modification of tissue, which comprises loading cells or cell-derived vesicles by mechanically shaking said cells or vesicles in the presence of an active substance intended to facilitate imaging, targeting, or biological modification, such active substance loaded cells upon injection into a host having a sufficiently long active life before disintegration or removal in the host to enable the intended imaging, targeting, or biological modification.
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Abstract
Methods are disclosed to rapidly form and load cells and cell-derived vesicles. Loaded materials can include imaging agents, drugs and magnetic particles. Methods are also presented to additionally target the loaded cells or vesicles, leading to new forms of imaging, treatment, diagnosis, and detection by a large number of techniques. The preparation and use of reduced sized cells that retain subset characteristics of the parent cell are also described.
83 Citations
61 Claims
- 1. A method of forming loaded cells, cell-derived vesicles or synthetic vesicles for facilitating in vivo imaging, targeting or biological modification of tissue, which comprises loading cells or cell-derived vesicles by mechanically shaking said cells or vesicles in the presence of an active substance intended to facilitate imaging, targeting, or biological modification, such active substance loaded cells upon injection into a host having a sufficiently long active life before disintegration or removal in the host to enable the intended imaging, targeting, or biological modification.
- 10. A method of forming loaded cells, cell-derived vesicles, or synthetic vesicles for facilitating in vivo imaging, targeting, or biological modification of tissue, which comprises loading cells or cell derived vesicles by first freezing and then thawing said cells or vesicles in the presence of an active substance intended to facilitate imaging, targeting or biological modification, such active substance loaded cells or vesicles upon injection into a host having a sufficient long active life before disintegration or removal in the host to enable the intended imaging, targeting, or biological modification.
- 16. A method of forming loaded cells, cell-derived vesicles, or synthetic vesicles for facilitating in vivo imaging, targeting or biological modification of tissue, which comprises loading cells or cell derived vesicles by passing said cells or vesicles through a porous material in the presence of an active substance intended to facilitate imaging, targeting, or biological modification such active substance loaded cells upon injection into a host having a sufficiently long active life before disintegration or removal in the host to enable the intended imaging, targeting, or biological modification.
- 22. A method of forming loaded cells, cell-derived vesicles, or synthetic vesicles for facilitating in vivo imaging, targeting or biological modification of tissue, which comprises loading cells or cell derived vesicles by fusing said cells or vesicles with liposomes or vesicles containing an active substance intended to facilitate imaging, targeting, or biological modification such active substance loaded cells upon injection into a host having a sufficiently long active life before disintegration or removal in the host to enable the intended imaging, targeting, or biological modification.
- 26. A method of enlarging the size of cells, cell-derived vesicles, or synthetic vesicles optionally loaded with an active substance intended to enable imaging, targeting, or biological modification by fusing two or more cells, cell-derived vesicles, or synthetic vesicles.
- 43. A method of facilitating CT, planar X-ray, or MRI imaging which comprises withdrawing blood from the host to be subjected to the X-ray and MRI imaging, loading the withdrawn blood with a contrast agent and reinjecting the product as obtained into said host, said contrast agent having a sufficiently long active life to enhance and perform the imaging before disintegration.
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58. The process of lysing cells, cell-derived vesicles, or synthetic vesicles loaded with a drug or agent (vesicular carrier) in an animal or human, thus releasing said drug or agent, comprising the steps of:
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1. loading said drug or agent in cells, cell-derived vesicles, or synthetic vesicles 2. choosing a membrane of said cells, cell-derived vesicles, or synthetic vesicles that has on its surface an antigen or molecule that can potentially activate the complement or immune system, or linking such an antigen or molecule to said membrane. 3. Administering said vesicular carriers to an animal or human. 4. The said vesicular carrier is targeted to a region in the body either naturally or by design. 5. Allowing the natural immune or complement system of the animal or human to respond resulting in lysis of said vesicular carrier and release of said drug or agent, or applying in an additional step an antibody or agent that binds specifically to said vesicular carrier that activates the immune or complement system resulting in lysis of said vesicular carrier and release of said drug or agent. - View Dependent Claims (59)
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Specification