Compositions and methods for generating antigen-binding units
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Abstract
The present invention provides non-single-chain antigen-binding units that is stabilized by leucine zipper sequences. The experimental design is particularly useful for generating and screening for Nsc Abus that remain the binding capabilities to their respective antigens within a cell. The present invention also provides recombinant polynucleotides, host cells and kits comprising the vectors. Further provided by the invention are methods of using the subject vectors.
13 Citations
76 Claims
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1-42. -42. (canceled)
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43. A non-single-chain antigen-binding unit comprising:
- (a) a light (L) chain polypeptide comprising a light (L) chain variable region fused to a first leucine zipper sequence;
(b) a heavy (H) chain polypeptide comprising a heavy (H) chain variable region fused to a second leucine zipper sequence;
wherein the L chain and the H chain polypeptides dimerize to form an antigen-binding site through an interaction between the first and second leucine zipper sequences. - View Dependent Claims (44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76)
- (a) a light (L) chain polypeptide comprising a light (L) chain variable region fused to a first leucine zipper sequence;
- 58. The host cell of 57, wherein the cell is eukaryotic.
Specification