Peptide Stabilization

• US 20070243558A1
• Filed: 05/09/2005
• Published: 10/18/2007
• Est. Priority Date: 05/10/2004
• Status: Abandoned Application

• Current Assignee: Biohit Oyj
• Original Assignee: Biohit Oyj
• Inventors: Suovaniemi, Osmo, Linanla, Auli
• Application Number:
  US11/579,004
• Publication Number:
  US 20070243558A1
• Time in Patent Office: Days
• US Class Current: 435/7.100
• CPC Class Codes: C07K 14/595 Gastrins; Cholecystokinins [CCK]
• CPC Class Codes: G01N 2333/595 Gastrins; Cholecystokinins [CCK]

Abstract

The present invention is directed to the use of DMSO for the stabilization of temperature sensitive proteins and peptides, such as gastrin, against degradation in a mammalian biological sample, such as a serum or plasma sample.

Claims

1. Use of DMSO for the stabilization of gastrin and gastrin related peptides against degradation in a mammalian biological sample.

2. The use according to claim 1, wherein the sample is a serum or plasma sample.

3. The use according to claim 1 or 2 for the stabilization of gastrin-17.

4. Method for stabilizing gastrin and gastrin related peptides peptides against degradation in a mammalian biological sample, according to which method a stabilizing amount of DMSO is added to the said sample.

5. The method according to claim 4 wherein the sample is a serum or plasma sample.

6. The method according to claim 4 or 5 for the stabilization of gastrin-17.

7. The method according to claim 6, wherein gastrin-17 is determined in a serum or plasma sample containing one or more analytes selected from the group consisting of pepsinogen I, pepsinogen II and Helicobacter pylori antibodies.