Genetically Engineered Pyrroloquinoline Quinone Dependent Glucose Dehydrogenase Comprising an Amino Acid Insertion
First Claim
1. A variant of the soluble form of EC 1.1.99.17 also known as PQQ-dependent soluble glucose dehydrogenase (s-GDH) said variant comprising at least one amino acid residue insertion between the amino acid positions corresponding to positions 428 and 429 of the s-GDH wild-type sequence known from A. calcoaceticus (SEQ ID NO:
- 2) and optionally in addition comprising one or more amino acid substitutions.
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Abstract
The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known from Acinetobacter calcoaceticus, to genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.
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Citations
22 Claims
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1. A variant of the soluble form of EC 1.1.99.17 also known as PQQ-dependent soluble glucose dehydrogenase (s-GDH) said variant comprising at least one amino acid residue insertion between the amino acid positions corresponding to positions 428 and 429 of the s-GDH wild-type sequence known from A. calcoaceticus (SEQ ID NO:
- 2) and optionally in addition comprising one or more amino acid substitutions.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
Specification