Droplet-based surface modification and washing
First Claim
1. A method of providing a droplet in contact with a surface with a reduced concentration of a substance, the method comprising:
- (a) providing a droplet microactuator comprising a surface in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume;
(b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and
(c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising;
(i) a droplet in contact with the surface having a decreased concentration of the substance relative to the starting concentration; and
(ii) a droplet which is separated from the surface.
4 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a droplet in contact with a surface with a reduced concentration of a substance is provided, wherein the method includes: (a) providing a droplet microactuator comprising a surface in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising: (i) a droplet in contact with the surface having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the surface.
-
Citations
76 Claims
-
1. A method of providing a droplet in contact with a surface with a reduced concentration of a substance, the method comprising:
-
(a) providing a droplet microactuator comprising a surface in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising; (i) a droplet in contact with the surface having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60)
-
- 10. The method of claim 10 further comprising releasing or resuspending one or more of the beads following step 1(c).
- 61. A method of modifying a surface on a droplet microactuator, the method comprising executing one or more droplet operations to bring a droplet comprising a surface-modifying agent into contact with the surface.
- 68. A droplet microactuator comprising a sample or reagent immobilized on a surface thereof and arranged such that a droplet on the droplet microactuator may contact the surface.
- 73. A method of removing a substance from a surface to which the substance is bound, the method comprising conducting one or more droplet operations to contact a droplet with the surface, the droplet comprising a solution for eluting the substance from the surface.
Specification