Protein crosslinkers, crosslinking methods and applications thereof
First Claim
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1. A method for crosslinking a biological fluid comprising combining a biological fluid with a crosslinker to covalently crosslink proteins endogenous to the biological fluid to form a crosslinked gel.
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Abstract
Some aspects of this disclosure relate to a method for crosslinking a biological fluid comprising combining a biological fluid with a crosslinker to covalently crosslink proteins endogenous to the biological fluid to form a crosslinked gel. Examples of a biological fluid are blood, plasma, or serum.
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58 Claims
- 1. A method for crosslinking a biological fluid comprising combining a biological fluid with a crosslinker to covalently crosslink proteins endogenous to the biological fluid to form a crosslinked gel.
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18. A low molecular weight precursor comprising:
a liquid crosslinker with a molecular weight of no more than about 2000 that comprises at least three functional groups that are strong electrophiles, wherein the crosslinker forms a melt at about 10°
C. to about 50°
C.- View Dependent Claims (19, 20, 21, 22, 23, 24, 25)
- 26. A method for forming a biomaterial in situ comprising combining a precursor with a solution of a crosslinker in an organic solvent to covalently crosslink the precursor to form a crosslinked gel.
- 37. A water soluble crosslinker comprising a purified preparation essentially free of water comprising a molecule that comprises a formula of R-(A)n wherein A is a strong electrophilic functional group, n is at least 2, and R has a molecular weight of about 40 to about 4000 and comprises an amide, secondary amine, or tertiary amine functional group.
- 45. A method of forming a biological material in a blood vessel with a lumen defined by walls of the blood vessel comprising combining a biological fluid with a crosslinker to covalently crosslink proteins endogenous to the biological fluid to form a crosslinked gel in situ on the walls of the blood vessel.
- 53. A biomaterial for drug delivery comprising a gel that comprises proteins covalently crosslinked with a synthetic crosslinker that is conformed to a wall of a blood vessel.
Specification