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METHODS FOR MONITORING THE EXPRESSION OF ALTERNATIVELY SPLICED GENES

  • US 20070248975A1
  • Filed: 05/04/2007
  • Published: 10/25/2007
  • Est. Priority Date: 04/25/2000
  • Status: Abandoned Application
First Claim
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1. A method for determining the sequence of a variant splicing product of an initial mRNA transcript composed of exons and introns, the method comprising:

  • employing different types of positive probes that hybridize with, and produce signals corresponding to, subsequences of the initial mRNA transcript in a sample solution;

    employing at least one type of negative control probe corresponding to each type of positive probe to produce negative-control-probe signals;

    detecting signals produced from positive probes in order to determine subsequences of the initial mRNA transcript present in a sample solution and construct an initial sequence of the variant splicing product; and

    detecting signals produced from the negative control probes to resolve ambiguities in the initial sequence of the variant splicing product.

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