INVERTED ORIENTATION FOR A MICROPLATE
First Claim
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1. A method of conducting amplification comprising:
- (a) loading a liquid polynucleotide sample into a well of a microplate;
(b) covering said well of said microplate with a cover such that said cover fluidly seals said liquid polynucleotide sample within said well;
(c) inverting said microplate such that said liquid polynucleotide sample contacts said cover; and
(d) thermocycling said microplate to promote amplification of polynucleotides in said liquid polynucleotide sample.
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Abstract
A method of conducting amplification comprising loading a liquid polynucleotide sample into a well of a microplate and covering the well of the microplate with a cover, such that the cover fluidly seals the liquid polynucleotide sample within the well. The method then comprises inverting the microplate such that the liquid polynucleotide sample contacts the cover; and thermocycling the microplate to promote amplification of polynucleotides in the liquid polynucleotide sample.
46 Citations
36 Claims
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1. A method of conducting amplification comprising:
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(a) loading a liquid polynucleotide sample into a well of a microplate;
(b) covering said well of said microplate with a cover such that said cover fluidly seals said liquid polynucleotide sample within said well;
(c) inverting said microplate such that said liquid polynucleotide sample contacts said cover; and
(d) thermocycling said microplate to promote amplification of polynucleotides in said liquid polynucleotide sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method of conducting polymerase chain reaction analysis comprising:
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(a) loading an assay into a well of a microplate;
(b) applying a cover to said well of said microplate such that said cover fluidly seals said assay within said well;
(c) inverting said microplate such that said assay contacts said cover; and
(d) thermocycling said microplate to promote amplification of said assay. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. A microplate comprising:
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a main body;
a plurality of wells disposed in said main body, said plurality of wells being sized to receive an assay; and
a cover operably coupled to said main body, said cover substantially sealing at least one of said plurality of wells when said plurality of wells contain said assay and said main body is inverted so that said assay is in contact with said sealing covering cover. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36)
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Specification