Aerobic Succinate Production in Bacteria
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Abstract
Methods of increasing yields of succinate using aerobic culture methods and a multi-mutant E. coli strain are provided. Also provided is a mutant strain of E. coli that produces high amounts of succinic acid.
12 Citations
32 Claims
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1-19. -19. (canceled)
- 20. A mutant strain of E. coli bacteria wherein said mutant strain comprises inactivation of sdhAB, ackA-pta, and poxB;
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27. A mutant strain of E. coli bacteria, wherein said mutant strain comprises:
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i) inactivation of sdhAB, ackA-pta, poxB, icd, and ii) overexpression of pepc.
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28. The mutant strain of claim 28, wherein the bacteria produces succinate at a ratio of at least 0.6:
- 1 moles succinate per mole glucose.
- View Dependent Claims (29)
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30. A mutant strain of E. coli bacteria, wherein said mutant strain comprises:
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i) inactivation ofsdhAB, ackA-pta, poxB, ptsG, icd, iclR, and ii) overexpression of pepc. - View Dependent Claims (31, 32)
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Specification