Metal-coated sorbents as a separation medium for HPLC of phosphorus-containing materials

• US 20070256976A1
• Filed: 04/10/2006
• Published: 11/08/2007
• Est. Priority Date: 04/10/2006
• Status: Abandoned Application

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Boyes, Barry, Moyer, Susanne, Martoscllo, James
• Application Number:
  US11/401,577
• Publication Number:
  US 20070256976A1
• Time in Patent Office: Days
• US Class Current: 210/639
• CPC Class Codes: B01D 15/3828 Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
• CPC Class Codes: B01J 20/0207 Compounds of Sc, Y or Lanthanides
• CPC Class Codes: B01J 20/0225 Compounds of Fe, Ru, Os, Co, Rh, Ir, Ni, Pd, Pt
• CPC Class Codes: B01J 20/103 comprising silica
• CPC Class Codes: B01J 20/28004 Sorbent size or size distribution, e.g. particle size
• CPC Class Codes: B01J 20/286 Phases chemically bonded to a substrate, e.g. to silica or to polymers
• CPC Class Codes: B01J 20/3204 Inorganic carriers, supports or substrates
• CPC Class Codes: B01J 20/3217 Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
• CPC Class Codes: B01J 20/3236 containing metal, other than zeolites, e.g. oxides, hydroxides, sulphides or salts
• CPC Class Codes: B01J 2220/54 Sorbents specially adapted for analytical or investigative chromatography
• CPC Class Codes: B01J 2220/58 Use in a single column
• CPC Class Codes: B01J 2220/64 In a syringe, pipette, e.g. tip or in a tube, e.g. test-tube or u-shape tube in columns B01J2220/58

Abstract

Methods for the separation of biological materials from a sample mixture are provided, using a support comprising metal coated onto a sorbent matrix. Methods using the support as a chromatographic column for separation are provided. The present disclosure also provides methods for making the metal-coated sorbent support.

Claims

1. A method for separating a target desired biological material from a sample, comprising:

contacting the sample to a support comprising a metal-coated sorbent matrix; and

capturing the target biological material on the support;

or

collecting the target biological material in the pass-through fraction.

2. The method of claim 1, wherein capturing the biological material on the support further comprises varying the pH to promote binding of the biological material to the support.

3. The method of claim 1, wherein the support is part of a column, spin tube, coated membrane, or powder.

4. The method of claim 1, wherein the sample comprises one or more or lipids, amino acids, nucleic acids, proteins, nucleotides, nucleosides, sugars, oligosaccharides, or mixtures thereof.

5. A method for separating one or more target biological materials from a sample mixture, comprising:

contacting the sample to a support comprising a metal-coated sorbent matrix; and

capturing one or more target biological materials on the support;

or

collecting one or more target biological materials in the pass-through fraction,

wherein one or more target materials are captured on the support or collected in the pass-through fraction, but other components of the sample mixture are not captured or collected.

6. The method of claim 5, wherein capturing one or more target biological materials on the support further comprises varying the pH to promote binding of the target biological materials to the support.

7. The method of claim 5, wherein the target biological material comprises one or more of a lipid, amino acid, peptide, protein, sugar, or oligosaccharide present in a sample mixture.

8. The method of claim 5, wherein the target biological material comprises a phosphopeptide.

9. A method for preparing a support for selective separation of a desired biological material from a sample, comprising the steps of:

providing a sorbent matrix;

selecting a metal for binding the desired biological material from a sample based on the affinity of the metal for the desired biological material; and

coating the sorbent matrix with the selected metal to form the support.

10. The method of claim 9, wherein the coating of the metal on the sorbent matrix is by covalent methods.

11. The method of claim 9, wherein the coating of the metal on the sorbent matrix is by non-covalent methods.

12. The method of claim 9, wherein the sorbent matrix comprises silica.

13. The method of claim 9, wherein the metal on the sorbent matrix is selected from the group consisting of Ni(II), Pt(II), and Ce(IV).