Detection and imaging of target tissue
First Claim
1. A method for high resolution imaging, comprising a) administering a targeted low resolution contrast agent and a targeted higher resolution contrast agent having an analogous target as the low resolution contrast agent to a subject, and allowing each contrast agent to accumulate in a target tissue;
- b) identifying the target tissue using a low resolution imaging technique to localize an accumulation of the low resolution contrast agent; and
c) obtaining a high resolution image of the target tissue using a high resolution imaging technique to localize an accumulation of the higher resolution contrast agent, thereby allowing the generation of a higher resolution image than that obtained by the use of the low resolution contrast agent alone.
3 Assignments
0 Petitions
Accused Products
Abstract
Methods for high resolution imaging of a suspected target tissue are encompassed by the invention. Such methods include administering low resolution and high resolution contrast agents specific to targeted cells or tissues. The contrast agents are allowed to bind to the target cells or accumulate in a target tissue. A low resolution imaging technique is used to localize an accumulation of the low resolution contrast agent in a target tissue. A high resolution image of the target tissue is then obtained to localize an accumulation of the higher resolution contrast agent, allowing the generation of a higher resolution image than that obtained by the use of the low resolution contrast agent alone. These methods may utilize nanoparticles optionally in an emulsion as a contrast agent.
-
Citations
32 Claims
-
1. A method for high resolution imaging, comprising
a) administering a targeted low resolution contrast agent and a targeted higher resolution contrast agent having an analogous target as the low resolution contrast agent to a subject, and allowing each contrast agent to accumulate in a target tissue; -
b) identifying the target tissue using a low resolution imaging technique to localize an accumulation of the low resolution contrast agent; and
c) obtaining a high resolution image of the target tissue using a high resolution imaging technique to localize an accumulation of the higher resolution contrast agent, thereby allowing the generation of a higher resolution image than that obtained by the use of the low resolution contrast agent alone. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
-
-
10. A method of delivering targeted contrast agents to a target tissue, which method comprises
a) administering a low resolution targeted contrast agent to a subject comprising a target tissue; -
b) administering a higher resolution targeted contrast agent to the subject, wherein the higher resolution contrast agent has an analogous target as the low resolution contrast agent; and
c) allowing the contrast agents to accumulate in the target tissue, to thereby deliver targeted contrast agents to the target tissue. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17)
-
- 18. A kit for the preparation of an emulsion of nanoparticles targeted to a tissue expressing a target moiety, which kit comprises at least one container that contains nanoparticles comprising a ligand specific for the target moiety and a linking moiety for coupling to a low resolution contrast agent and/or a higher resolution contrast agent, at least one container that contains the low resolution contrast agent, and at least one container that contains the higher resolution contrast agent.
-
23. A kit for the preparation of an emulsion of nanoparticles targeted to a tissue expressing a target moiety, which kit comprises at least one container that contains nanoparticles comprising a linking moiety for coupling to a ligand specific for the target moiety, at least one container that contains a ligand specific for the target moiety, at least one container that contains a low resolution contrast agent, and at least one container that contains a higher resolution contrast agent.
- 24. A kit for high resolution imaging, comprising at least one container that contains a targeted low resolution contrast agent, at least one container that contains a higher resolution contrast agent, and instructions means for use.
- 30. A kit for high resolution imaging, comprising at least one container that contains halocarbon-based nanoparticles comprising a ligand specific for a target moiety, wherein the nanoparticles are coupled to a higher resolution contrast agent, and instructions means for use.
Specification