In vitro sorting method
First Claim
1. A method for screening a compound or compounds capable of modulating the activity of a gene product, comprising the steps of:
- (a) preparing a repertoire of genetic elements encoding the gene product;
(b) expressing the genetic elements to produce their respective gene products and linking the gene products to the genetic elements encoding them;
(c) compartmentalising the genetic elements into microcapsules;
(d) sorting the genetic elements which produce the gene product(s) having a desired activity; and
(e) contacting a gene product having the desired activity with the compound or compounds and monitoring the modulation of an activity of the gene product by the compound or compounds.
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Abstract
The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising of the steps of: (a) compartmentalising genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within the microcapsules; (c) sorting the genetic elements which produce the gene product having a desired activity. The invention enables the in vitro evolution of nucleic acids by repeated mutagenesis and iterative applications of the method of the invention.
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Citations
14 Claims
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1. A method for screening a compound or compounds capable of modulating the activity of a gene product, comprising the steps of:
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(a) preparing a repertoire of genetic elements encoding the gene product;
(b) expressing the genetic elements to produce their respective gene products and linking the gene products to the genetic elements encoding them;
(c) compartmentalising the genetic elements into microcapsules;
(d) sorting the genetic elements which produce the gene product(s) having a desired activity; and
(e) contacting a gene product having the desired activity with the compound or compounds and monitoring the modulation of an activity of the gene product by the compound or compounds. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification