RAPID LOCALIZED CELL TRAPPING ON BIODEGRADABLE POLYMERS

• US 20070259382A1
• Filed: 11/10/2006
• Published: 11/08/2007
• Est. Priority Date: 11/22/2005
• Status: Abandoned Application

• Current Assignee: University of Iowa Research Foundation
• Original Assignee: University of Iowa Research Foundation
• Inventors: SALEM, ALIASGER
• Application Number:
  US11/558,540
• Publication Number:
  US 20070259382A1
• Time in Patent Office: Days
• US Class Current: 435/7.500
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00382 Stamping
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00743 Cells

Abstract

The present invention provides methods and compositions for cell-based arrays. A biotin-avidin system for the attachment of cells onto a surface also employs biodegradable polymers onto which avidin is disposed in a predetermined pattern.

Claims

1. A method of immobilizing cells comprising:

(a) providing a surface comprising avidin, wherein in avidin is disposed in a pre-determined pattern; and

(b) contacting said surface comprising avidin with a biotinylated cell population,

whereby said cell population is immobilized to said surface according to said predetermined pattern.

2. The method of claim 1, wherein said avidin is attached to said surface by interaction with biotin bound to said surface.

3. The method of claim 2, wherein said biotin is bound to said surface through a block copolymer, which is in turn bound to said surface.

4. The method of claim 3, wherein said block copolymer is biodegradable.

5. The method of claim 2, wherein said block copolymer is silk, elastin, chitin, chitosan, poly(d-hydroxy acid), poly(anhydrides), and poly(athoesters).

6. The method of claim 3, further comprising a step, prior to step (a), of applying said block copolymer to said surface.

7. The method of claim 1, further comprising a step, prior to step (a), of applying said avidin to said surface.

8. The method of claim 7, wherein applying avidin to said surface comprises microfluidic networking.

9. The method of claim 8, wherein said microfluidic networking employs a poly-dimethylsiloxane stamp.

10. The method of claim 1, further comprising a step, prior to step (b), of biotinylating said cell.

11. The method of claim 10, wherein biotinylating said cell comprises treating said cell with sodium periodate to generate a cell comprising non-native aldehydes, and reacting said cell comprising non-native aldehydes with biotin-hydrazine.

12. The method of claim 1, wherein said surface further comprises block copolymer lacking avidin in areas outside said predetermined pattern.

13. The method of claim 12, wherein said block copolymer lacking avidin is biodegradable.

14. The method of claim 12, wherein said cell population is bound to said predetermined pattern at levels about 10-fold, about 15-fold, about 20-fold or about 25-fold higher than outside said predetermined pattern.

15. The method of claim 1, wherein said cell population is naturally adherent.

16. The method of claim 1, wherein said cell population is naturally non-adherent.

17. The method of claim 1, wherein said cell population is a fibroblast population, an endothelial cell population, a neuronal cell population, or an epithelial cell population.

18. The method of claim 1, wherein said surface comprises silicon, plastic, glass or paper.

19. The method of claim 1, wherein said surface is disposed in a culture dish, a biochip, a column, or a filter.

20. The method of claim 1, wherein said predetermined pattern comprises cells dispersed in a line, a square, a rectangle, a circle, an oval or combinations thereof.

21. The method of claim 1, wherein said cell population, when bound to said surface, is 85% viable.

22. A device comprising:

(a) a surface;

(b) a biotinylated copolymer bound to said surface;

(c) avidin bound to said biotinylated copolymer in a predetermined pattern; and

(d) a biotinylated cell population bound to said avidin.