Blood Hemolysis Analyzer
First Claim
1. A system for measuring the concentration of at least one cell-free hemoglobin derivative in a sample containing hemoglobin derivatives contained within erythrocytes, the system comprising:
- a light source configured to emit light;
a sample block configured to allow the light to pass through the sample wherein, a first portion of the light, having a wavelength of about 390-460 nm, is absorbed by the cell-free hemoglobin derivatives and a second portion is absorbed by the hemoglobin derivatives contained within erythrocytes;
at least one absorption detector configured to detect the light absorption within a wavelength range of about 390-460 nm that is absorbed by the sample; and
a processor configured to compare the change in absorption of the light by the sample at least at two wavelengths and thereby determine the concentration of the cell-free hemoglobin derivatives.
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Accused Products
Abstract
Systems and methods for determining the concentration of hemoglobin derivatives in bodily fluids include devices for measuring and comparing the absorption of electromagnetic radiation by cellular and cell-free hemoglobin at two or more wavelengths in the Soret region. Systems and methods for determining erythrocyte membrane fragility include devices for measuring the concentration of at least one cell-free hemoglobin derivative, and using the absorption properties of cellular and cell-free hemoglobin derivatives in the Soret region.
26 Citations
22 Claims
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1. A system for measuring the concentration of at least one cell-free hemoglobin derivative in a sample containing hemoglobin derivatives contained within erythrocytes, the system comprising:
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a light source configured to emit light; a sample block configured to allow the light to pass through the sample wherein, a first portion of the light, having a wavelength of about 390-460 nm, is absorbed by the cell-free hemoglobin derivatives and a second portion is absorbed by the hemoglobin derivatives contained within erythrocytes; at least one absorption detector configured to detect the light absorption within a wavelength range of about 390-460 nm that is absorbed by the sample; and a processor configured to compare the change in absorption of the light by the sample at least at two wavelengths and thereby determine the concentration of the cell-free hemoglobin derivatives. - View Dependent Claims (2, 3, 4)
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5. A method for directly measuring the concentration of at least one cell-free hemoglobin derivative in a sample comprising:
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providing;
a light source, a sample comprising erythrocytes, an absorption detector capable of detecting light in the spectral range of about 390-460 nm;exposing the sample to the light source; detecting the absorption by the sample in the spectral range of about 390-460 nm; and comparing the absorption spectrum of the sample at least at two wavelengths and thereby determining the concentration of at least one cell-free hemoglobin derivative in the sample. - View Dependent Claims (6, 7, 8, 9, 10, 11)
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12. A system for measuring erythrocyte membrane fragility in a sample comprising:
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a light source configured to emit light; an erythrocyte stressor configured to apply a defined stress to the erythrocyte membranes; a sample block configured to allow the light to pass through the sample wherein a first portion of the light having a wavelength of about 390-460 nm is absorbed by the cell-free hemoglobin derivatives in the sample and a second portion is absorbed by the hemoglobin derivatives contained within erythrocytes; at least one absorption detector configured to detect the light absorption within a wave length range of about 390-460 nm that is absorbed by the sample; and a processor configured to compare the change in absorption of the light by the sample at least at two wavelengths and thereby determine the concentration of the cell-free hemoglobin derivatives. - View Dependent Claims (13, 14, 15)
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16. A method for the measurement of erythrocyte membrane fragility in a sample comprising:
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providing a light source, a sample comprising erythrocytes, an erythrocyte stressor configured to stress the erythrocytes in the sample, and an absorption detector configured to detect light absorption within the wavelength range of about 390-460 nm; exposing the stressed erythrocytes in the sample to the light source; and comparing the absorption spectrum of the cell-free hemoglobin derivatives in the sample to the flatted absorption spectrum of the hemoglobin derivatives in erythrocytes. - View Dependent Claims (17, 18, 19, 20, 21, 22)
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Specification