Aerosol jet deposition method and system for creating a reference region/sample region on a biosensor

• US 20070264155A1
• Filed: 05/09/2006
• Published: 11/15/2007
• Est. Priority Date: 05/09/2006
• Status: Abandoned Application

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Brady, Michael, Yan, Yongsheng, Peterson, Richard, Peanasky, John
• Application Number:
  US11/431,226
• Publication Number:
  US 20070264155A1
• Time in Patent Office: Days
• US Class Current: 422/58
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/0036 Nozzles
• CPC Class Codes: B01J 2219/00436 Maskless processes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00662 Two-dimensional arrays within two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00693 Means for quality control
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01L 2200/0636 Focussing flows, e.g. to laminate flows
• CPC Class Codes: B01L 2300/0819 : Microarrays; Biochips
• CPC Class Codes: B01L 3/0268 : using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries

Abstract

A method and deposition device are described herein that use an aerosol jet direct write technique to create non-binding reference region(s) and/or binding sample region(s) within a single well or multiple wells of a microplate, or on a single or multiple biosensors of an unassembled bottom insert.

Claims

1. A method for preparing a biosensor which has a surface with a sample region and a reference region, said method comprising the step of:

utilizing an aerosol jet deposition technique to create the reference region and/or the sample region on the surface of said biosensor.

2. The method of claim 1, wherein said reference region and said sample region are created on the surface of said biosensor by performing the following steps:

creating the reference region by using the aerosol jet deposition technique to deposit a deactivating agent on a first predetermined area of the surface; and

creating the sample region by depositing a reactive agent on a second predetermined area of the surface.

3. The method of claim 2, wherein said sample region is created on the surface of said biosensor by using said aerosol jet deposition technique.

4. The method of claim 1, wherein said reference region and said sample region are created on the surface of said biosensor by performing the following steps:

creating the sample region by coating the surface with a reactive agent; and

creating the reference region by using the aerosol jet deposition technique to deposit a deactivating agent on a predetermined area of the coated reactive surface.

5. The method of claim 4, wherein said sample region is created on the surface of said biosensor by using said aerosol jet deposition technique to deposit the reactive agent on the surface.

6. The method of claim 4, wherein said step of creating the reference region by using the aerosol jet deposition technique includes the steps of:

atomizing the deactivating agent;

using a carrier gas to transport said atomized deactivating agent;

injecting a sheath gas around said carrier gas and said atomized deactivating agent; and

directing said sheath gas, said carrier gas and said atomized deactivating agent towards the predetermined area on the coated surface.

7. The method of claim 4, wherein said step of creating the reference region by using the aerosol jet deposition technique includes the step of:

controlling a thickness/uniformity/spreading of the deposited deactivating agent.

8. The method of claim 1, further comprising the step of exposing the surface to target molecules where the target molecules bind to the sample region created on the surface and do not bind to the reference region created on the surface.

9. The method of claim 1, further comprising the step of interrogating said biosensor such that a sample signal from the sample region is used to detect a chemical/biomolecular binding event and a reference signal from the reference region is used to reference out from the sample signal spurious changes that adversely affect the detection of the chemical/biomolecular binding event.

10. The method of claim 1, further comprising the step of interrogating said biosensor such that a sample signal from the sample region is used to perform a cell assay and a reference signal from the reference region is used to reference out from the sample signal spurious changes that adversely affect the cell assay.

11. A deposition device, comprising:

an atomizing chamber in which an agent is atomized and in which a carrier gas is inserted to transport the atomized agent;

a deposition head that receives the carrier gas and the atomized agent and injects a sheath gas around the carrier gas and the atomized agent; and

a nozzle that directs the sheath gas, the carrier gas and the atomized agent towards a predetermined area on a surface of a biosensor.

12. The deposition device of claim 11, further comprising a platform which supports and moves the biosensor so the atomized agent can be deposited on the biosensor.

13. The deposition device of claim 11, further comprising a shuttering mechanism that moves to either permit or block said sheath gas, said carrier gas and said atomized agent emitted from said nozzle from reaching the surface of the biosensor.

14. The deposition device of claim 11, further comprising a processor that ensures a desired thickness/uniformity/line width of the atomized agent is deposited/patterned onto the biosensor.

15. The deposition device of claim 11, wherein said agent is a deactivating agent which is deposited so as to form a reference region on the biosensor.

16. The deposition device of claim 11, wherein said agent is a reactive agent which is deposited so as to form a sample region on the biosensor.

17. A biosensor, comprising:

a surface that has a reference region and/or a sample region which was created in part by using an aerosol jet deposition technique.

18. The biosensor of claim 17, wherein the reference region and the sample region were created on said surface by performing the following steps:

creating the reference region by using the aerosol jet deposition technique to deposit a deactivating agent on a first predetermined area of the surface; and

creating the sample region by depositing a reactive agent on a second predetermined area of the surface.

19. The biosensor of claim 18, wherein said sample region is created on said surface by using said aerosol jet deposition technique.

20. The biosensor of claim 17, wherein said reference region and said sample region are created on said surface by performing the following steps:

creating the sample region by coating the surface with a reactive agent; and

creating the reference region by using the aerosol jet deposition technique to deposit a deactivating agent on a predetermined area of the coated reactive surface.

21. The biosensor of claim 20, wherein said sample region is created on said surface by using said aerosol jet deposition technique to deposit the reactive agent on the surface.

22. The biosensor of claim 20, wherein said deposited deactivating agent has a thickness in a range of 1 nm-3000000 nm.

23. The biosensor of claim 20, wherein said deposited deactivating agent has a line width that is ≧

10 μ

m.

24. The biosensor of claim 20, wherein said deposited deactivating agent has a droplet size which is 1-25 μ

m.

25. The biosensor of claim 17, wherein said surface has more than one reference region and/or more than one sample region.

26. The biosensor of claim 17, wherein said surface is a slide.

27. The biosensor of claim 17, wherein said surface is a bottom of a well in a microplate.

28. The biosensor of claim 17, wherein said surface is an unassembled bottom insert which is used to make a microplate.