Nanosensors

• US 20070264623A1
• Filed: 06/15/2005
• Published: 11/15/2007
• Est. Priority Date: 06/15/2004
• Status: Abandoned Application

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: Chen, Chuo, Fang, Ying, Wang, Wayne, Lieber, Charles, Lin, Keng-Hui
• Application Number:
  US11/629,722
• Publication Number:
  US 20070264623A1
• Time in Patent Office: Days
• US Class Current: 435/4
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: G01N 27/4146 involving nanosized elements, e.g. nanotubes, nanowires
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

The present invention generally relates to nanoscale wires for use in determining analytes suspected to be present in a sample, especially in connection with determining information about a sample containing, or suspected of containing, two or more analytes. For example, the invention can involve a competitive, uncompetitive, or non-competitive binding assay including a nanoscale wire to a sample containing a species able to interact with the retain entity to produce a product, where the sample also contains or is suspected of containing a second species able to interact with the reaction entity to prevent production of the product resulting from interaction of the first species and the reaction entity. Based upon determination of production of the product, determination of the second species in the sample can be made. In one set of embodiments, nanoscale wires can be used that have been functionalized at their surface, and/or in close proximity to their surface, for example, by immobilizing a protein or an enzyme relative to the nanoscale wire. Functionalization may permit interaction of the nanoscale wire with various analytes, and such interaction may induce a determinable change in a property of the nanoscale wire. Determination of two or more analytes, o one analyte and the suspected presence of another analyte can involve, for example, binding species to a protein or an enzyme immobilized relative to the nanoscale wire. Other aspects of the invention include assays, sensors, detectors, and/or other devices that include functionalized nanoscale wires, methods of making and/or using functionalized nanoscale wires (for example, in drug screening or high throughput screening) and the like.

Claims

1. A system, comprising:

a sample exposure region comprising a reaction entity associated with a nanoscale wire; and

a first species and a second species different from the first species, each within the sample exposure region, wherein each of the first and second species is able to interact with the reaction entity or to affect interaction of the reaction entity with the other species.

2. The system of claim 1, wherein the first species is able to interact with the reaction entity to produce a product, and the second species is able to interact with the reaction entity to prevent or inhibit production of the product resulting from interaction of the first species and the reaction entity.

3. The system of claim 1, wherein the first species is able to interact with the reaction entity to produce a product, and the second species is a drug candidate able to interact with the first species, the reaction entity, or both, to affect interaction of the first species and the reaction entity.

4. The system of claim 1, wherein the first species and the second species competitively bind to the reaction entity.

5. The system of claim 1, wherein the first species and the second species uncompetitively bind to the reaction entity.

6. The system of claim 1, wherein the first species and the second species noncompetitively bind to the reaction entity.

7. The system of claim 1, wherein an interaction between the reaction entity and at least one of the first species and the second species causes a detectable change in a property of the nanoscale wire.

8. The system of claim 1, wherein an interaction between the reaction entity and the first species causes a first detectable change in a property of the nanoscale wire, and an interaction between the reaction entity and the second species causes a second detectable change in a property of the nanoscale wire, the first detectable change being different from the second detectable change.

9. The system of claim 1, wherein the reaction entity comprises a binding partner of at least one of the first species and the second species.

10. The system of claim 9, wherein the binding partner is non-specific.

11. The system of claim 9, wherein the binding partner is specific.

12. The system of claim 9, wherein the binding partner comprises a biomolecular receptor.

13. The system of claim 12, wherein the biomolecular receptor includes a moiety selected from the group consisting of DNA, a fragment of DNA, an antibody, an antigen, a protein, an enzyme, and combinations thereof.

14. The system of claim 1, wherein the reaction entity includes an entity selected from the group consisting of a nucleic acid, an antibody, a sugar, a carbohydrate, a protein, and combinations thereof.

15. The system of claim 1, wherein the reaction entity comprises a protein.

16. The system of claim 1, wherein the reaction entity comprises an enzyme.

17. The system of claim 1, wherein the reaction entity comprises a catalyst.

18. The system of claim 1, wherein the reaction entity comprises a polymer.

19. The system of claim 1, wherein the reaction entity is fastened to the nanoscale wire.

20. The system of claim 1, wherein the reaction entity is positioned within 100 nanometers of the nanoscale wire.

21. The system of claim 1, wherein the reaction entity is positioned within 50 nanometers of a nanoscale wire.

22. The system of claim 1, wherein the reaction entity is positioned within 10 nanometers of a nanoscale wire.

23. The system of claim 1, wherein the reaction entity is positioned within 5 nanometers of the nanoscale wire.

24. The system of claim 1, wherein the reaction entity is positioned within 3 nanometers of the nanoscale wire.

25. The system of claim 1, wherein the reaction entity is positioned within 1 nanometer of the nanoscale wire.

26. The system of claim 1, wherein the reaction entity is attached to the nanoscale wire through a linker.

27. The system of claim 1, wherein the reaction entity is directly attached to the nanoscale wire.

28. The system of claim 1, the reaction entity being positioned relative to the nanoscale wire such that it is electrically coupled to the nanoscale wire, wherein a detectable interaction between the reaction entity and at least one of the first and second species causes a detectable change in an electrical property of the nanoscale wire.

29. The system of claim 1, wherein the nanoscale wire comprises a semiconductor.

30. The system of claim 29, wherein the semiconductor nanoscale wire comprises silicon.

31. The system of claim 1, wherein the nanoscale wire is a nanotube.

32. The system of claim 31, wherein the nanotube includes a carbon nanotube.

33. The system of claim 1, wherein the nanoscale wire is a nanowire.

34. The system of claim 1, wherein the nanoscale wire is unmodified.

35. The system of claim 1, wherein the nanoscale wire is positioned on the surface of a substrate.

36. The system of claim 1, constructed and arranged to receive a fluidic sample in the sample exposure region.

37. The system of claim 36, wherein the sample is a gas stream.

38. The system of claim 36, wherein the sample is a liquid.

39. The system of claim 1, further comprising a detector constructed and arranged to determine a property associated with the nanoscale wire.

40. The system of claim 39, wherein the property is an electrical property.

41. The system of claim 39, wherein the property is an electromagnetic property.

42. The system of claim 39, where the property is a light emission property.

43. The system of claim 1, wherein the sample exposure region comprises a microchannel.

44. The system of claim 1, wherein the nanoscale wire is one of plurality of nanoscale wires, each of the plurality of nanoscale wires being doped with different concentrations of a dopant.

45. The system of claim 1, wherein the nanoscale wire is one of a plurality of nanoscale wires comprising a sensor.

46. The system of claim 45, wherein the plurality of nanoscale wires comprises at least 10 nanoscale wires.

47. The system of claim 45, wherein the plurality of nanoscale wires are arranged in parallel and addressed by a single pair of the electrodes.

48. The system of claim 45, wherein the plurality of nanoscale wires are arranged in parallel to each other and addressed individually by multiple pairs of electrodes.

49. The system of claim 45, wherein the plurality of nanoscale wires are different, each capable of detecting a different analyte.

50. The system of claim 45, wherein the plurality of nanoscale wires are oriented randomly.

51. A method, comprising an act of:

exposing a reaction entity associated with a nanoscale wire to a sample containing a first species and containing or suspected of containing a second species different from the first species, each species able to interact with the reaction entity and/or able to affect the interaction of the other species with the reaction entity.

52. The method of claim 51, wherein the first species is able to interact with the reaction entity to produce a product, and the second species is able to interact with the reaction entity to prevent or inhibit production of the product resulting from interaction of the first species and the reaction entity, and based upon determination of production of the product, determining the second species in the sample.

53. The method of claim 51, wherein the first species and the second species can competitively bind to the reaction entity.

54. The method of claim 51, wherein the first species and the second species can uncompetitively bind to the reaction entity.

55. The method of claim 51, wherein the first species and the second species can noncompetitively bind to the reaction entity.

56. The method of claim 51, wherein the first species is able to interact with the reaction entity, and the sample is known to contain the second species and the second species is a drug candidate suspected of having the ability to affect the interaction of the first species and the reaction entity, the method comprising determining the ability of the second species to affect the interaction.

57. The method of claim 51, further comprising determining a property associated with the nanoscale wire.

58. The method of claim 57, wherein the property is an electrical property.

59. The method of claim 51, wherein the nanoscale wire comprises a semiconductor.

60. The method of claim 59, wherein the semiconductor nanoscale wire comprises silicon.

61. The method of claim 51, wherein the nanoscale wire is a nanotube.

62. The method of claim 61, wherein the nanotube includes a carbon nanotube.

63. The method of claim 51, wherein the nanoscale wire is a nanowire.

64. The method of claim 51, wherein the reaction entity comprises a binding partner of at least one of the first species and the second species.

65. The method of claim 64, wherein the binding partner is non-specific.

66. The method of claim 64, wherein the binding partner is specific.

67. The method of claim 51, wherein the reaction entity comprises a protein.

68. The method of claim 51, wherein the reaction entity comprises an enzyme.

69. The method of claim 51, wherein the reaction entity comprises a catalyst.

70. The method of claim 51, wherein the reaction entity comprises a polymer.

71. The method of claim 51, wherein the reaction entity is fastened to the nanoscale wire.

72. The method of claim 51, wherein the reaction entity is positioned within 5 nanometers of the nanoscale wire.

73. The method of claim 51, wherein the reaction entity is attached to the nanoscale wire through a linker.

74. The method of claim 51, the reaction entity being positioned relative to the nanoscale wire such that it is electrically coupled to the nanoscale wire.

75. A method, comprising acts of:

exposing a nanoscale wire to an analyte; and

determining a binding constant between the analyte and the nanoscale wire.