NANOPOROUS MEMBRANE IMMUNOSENSOR
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Accused Products
Abstract
A sensor for a selected analyte in a test sample has (a) a semipermeable membrane with pores for retaining the analyte, where the membrane has been chemically modified by attachment of membrane modifiers; (b) immunoassay labels which have label binding ligands where these label binding ligands will have a binding affinity for the membrane modifiers in the presence of the analyte, and a measurably different binding affinity for the membrane modifiers in the absence of the analyte; and (c) a label detecting system, for detecting the presence of the labels on the membrane.
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Citations
36 Claims
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1-14. -14. (canceled)
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15. A sensor for independently detecting a plurality of analytes in a test solution, comprising:
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a test vessel;
a semipermeable membrane with pores for retaining the analyte, dividing said test vessel into a first volume and a second volume, wherein the surface of said membrane is chemically modified by attachment of at least two distinct membrane surface modifiers on at least a side facing said first volume, wherein said at least two distinct membrane surface modifiers are patterned into an array of distinct regions on said membrane surface;
at least two distinct groups of immunoassay labels disposed within said first volume, wherein each of said groups of immunoassay labels has distinct label binding ligands where these label binding ligands will have a binding affinity for one of said distinct membrane surface modifiers in the presence of the analyte, and a measurably different binding affinity for said one of said distinct membrane surface modifiers in the absence of the analyte;
a pressure source, for driving said test solution from said first volume into said second volume; and
a label detecting system, for detecting the presence or absence of said labels in each of said regions on said membrane.
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16-25. -25. (canceled)
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26. A sensor for independently detecting a plurality of analytes in a test solution, comprising:
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a test vessel;
a semipermeable membrane with pores for retaining the analyte, dividing said test vessel into a first volume and a second volume, said pores are selected to prevent the analyte from passing into or through said semipermeable membrane;
wherein said membrane is chemically modified by attachment of at least two distinct membrane modifiers on at least a side facing said first volume but not within said pores;
wherein said at least two distinct membrane modifiers are patterned into an array of distinct regions on said membrane;
at least two distinct groups of assay labels disposed within said first volume, wherein each of said groups of assay labels has distinct label modifiers, said label modifiers having a binding affinity for one of said distinct membrane modifiers in the presence of the analyte, and a measurably different binding affinity for said one of said distinct membrane modifiers in the absence of the analyte;
a pressure source, for driving said test solution from said first volume into said second volume; and
a label detecting system, for detecting the presence or absence of said labels in each of said regions on said membrane. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35)
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36-45. -45. (canceled)
Specification