Microflow path system

• US 20070267336A1
• Filed: 05/17/2007
• Published: 11/22/2007
• Est. Priority Date: 05/22/2006
• Status: Abandoned Application

• Current Assignee: Sony Corporation (Sony Group Corp.)
• Original Assignee: Sony Corporation (Sony Group Corp.)
• Inventors: Abe, Tomoteru, Ohnishi, Michihiro
• Application Number:
  US11/798,890
• Publication Number:
  US 20070267336A1
• Time in Patent Office: Days
• US Class Current: 210/198.200
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/084 Passive control of flow resistance
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: B01L 3/561 Tubes; Conduits in general F16L
• CPC Class Codes: G01N 30/603 retaining the stationary phase, e.g. Frits

Abstract

A microflow path system has a column layer of at least 0.5 mm in internal diameter. The column layer includes a hybrid-forming section and a fluid-supply promoting section arranged in a region downstream of the hybrid-forming section. The hybrid-forming section is packed with beads to provide a bead bed length of not longer than 4 mm. The fluid-supply promoting section serves to contribute to an increase in a flow rate of a fluid supply.

Claims

1. A microflow path system having a column layer of at least 0.5 mm in internal diameter, said column layer comprising:

a hybrid-forming section packed with beads to provide a bead bed length of not longer than 4 mm; and

a fluid-supply promoting section arranged in a region downstream of said hybrid-forming section to contribute to an increase in a flow rate of a fluid supply.

2. The microflow path system according to claim 1, wherein a sample fluid filtered through a filter of not greater than 0.65 μ

m in pore size is supplied.

3. The microflow path system according to claim 1, wherein said fluid-supply promoting section is packed with perfusion chromatography particles.

4. The microflow path system according to claim 1, further comprising a device configured to perform depressurization on a downstream of said fluid-supply promoting section.

5. The microflow path system according to claim 1, wherein a nucleic acid is immobilized on said beads via a linker having a same base sequence.

6. The microflow path system according to claim 5,

wherein said same base sequence of said linker is poly(T), and

in said hybrid-forming section, there are conducted a first hybrid formation that said poly(T) and an mRNA having a poly(A) tail extension in a total RNA sample complementarily bind each other and a second hybrid formation that said mRNA and a probe nucleic acid complementarily bind each other.