Method for Producing Bioassay Plate by Stacking Two Substrates Together and Bioassay Plate

• US 20070269344A1
• Filed: 09/27/2004
• Published: 11/22/2007
• Est. Priority Date: 10/03/2003
• Status: Abandoned Application

• Current Assignee: Sony Corporation (Sony Group Corp.)
• Original Assignee: Sony Corporation (Sony Group Corp.)
• Inventors: Aki, Yuichi, Ohnishi, Michihiro, Inagaki, Minoru
• Application Number:
  US10/574,214
• Publication Number:
  US 20070269344A1
• Time in Patent Office: Days
• US Class Current: 422/68.100
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

A bioassay plate is provided with a pair of opposing electrodes in a reaction region, and by imposing a predetermined electric field on the reaction region, the bioassay plate makes it possible to perform high-order structural adjustment, migration, immobilization and the like of a substance as desired. A first substrate (11) is provided with a detection well (X), which is in turn equipped at least with a reaction region (R) for providing a place of interaction between the substances and also with a first electrode (E₁₁) arranged facing the reaction region (R). A second substrate (12) is provided at least with a second electrode (E₁₂) which can impose an electric field on the reaction region (R) in association with the first electrode (E₁₁). The present invention provides a bioassay plate (1) formed of these two substrates (11), (12) stacked together such that the first electrode (E₁₁) and the second electrode (E₁₂) are located opposite to each other, and also a production method of the plate (1).

Claims

1. A bioassay plate comprising a first substrate and a second substrate stacked together,

said first substrate being provided at least with a reaction region for providing a place of an interaction between substances in a liquid phase and also with a first electrode arranged to face said reaction region, and

said second substrate being provided at least with a common second electrode formed to be located oppose said first electrode.

2. The bioassay plate according to claim 1, wherein an axis of opposition between said first electrode and said common second electrode is perpendicular to a bottom wall of said reaction region.

3. The bioassay plate according to claim 1, wherein a light source is arranged at a position of said first electrode, and a light-receiving portion is arranged at a position of said common second electrode.

4. The bioassay plate according to claim 1, wherein a light-receiving portion is arranged at a position of said first electrode, and a light source is arranged at a position of said common second electrode.

11. The bioassay plate according to claim 1, wherein said first substrate as defined in claim 1 and said second substrate as defined in claim 1 are stacked together, said first substrate carries thereon plural detecting portions, and said second substrate is provided with a common second electrode for establishing a relation of opposing electrodes with first electrodes as defined in claim 1 and arranged in said respective plural detecting portions as a unit.

12. The bioassay plate according to claim 1, wherein disk-shaped, said first substrate as defined in claim 1 and said second substrate as defined in claim 1 are stacked together, said first substrate carries thereon plural detecting portions arranged in a form of radial arrays from a center of said substrate, and said second substrate is provided with a strip-shaped or line-shaped, common second electrode as defined in claim 1 and formed extending over some or all of said detecting portions belonging to one of said radial arrays.

13. The bioassay plate according to claim 1, wherein disk-shaped, said first substrate as defined in claim 1 and said second substrate as defined in claim 1 are stacked together, said first substrate carries thereon plural detecting portions arranged in a form of concentric arrays, and said second substrate is provided with a strip-shaped or line-shaped, said common second electrode as defined in claim 1 and formed extending over some or all of said detecting portions belonging to one of said concentric arrays.

14. The bioassay plate according to claim 1, wherein disk-shaped, said first substrate as defined in claim 1 and said second substrate as defined in claim 1 are stacked together, said first substrate carries thereon plural detecting portions arranged in a form of a spiral array, and said second substrate is provided with a strip-shaped or line-shaped, said common second electrode as defined in claim 1 and formed extending over some or all of said detecting portions belonging to one spiral array.

15. The bioassay plate according to claim 1, wherein said first electrode and said common second electrode are electrodes for forming an alternating electric field between said first electrode and said common second electrode.

5. A method for producing a bioassay plate, which comprises providing:

a first substrate having a detecting portion provided at least with a reaction region for providing a place of an interaction between substances and also with a first electrode arranged to face said reaction region, and

a second substrate provided at least with a second electrode which permits imposition of an electric field to said reaction region in association with said first electrode; and

stacking these two substrates together such that said first electrode and said second electrode are located opposite each other.

6. The method according to claim 5, wherein a first substrate as defined in claim 5 and a second substrate as defined in claim 5 are stacked together, said first substrate carries thereon an array of plural detecting portions as defined in claim 5, and said second substrate is provided with a common second electrode for establishing a relation of opposing electrodes with first electrodes as defined in claim 5 and arranged in said respective plural detecting portions as a unit.

7. The method according to claim 5, wherein disk-shaped, said first substrate as defined in claim 5 and said second substrate as defined in claim 5 are stacked together, said first substrate carries thereon plural detecting portions as defined in claim 5 and arranged in a form of radial arrays from a center of said substrate, and said second substrate is provided with a strip-shaped or line-shaped, common second electrode as defined in claim 5 and formed extending over some or all of said detecting portions belonging to one of said radial arrays.

8. The method according to claim 5, wherein disk-shaped, said first substrate as defined in claim 5 and said second substrate as defined in claim 5 are stacked together, said first substrate carries thereon plural detecting portions as defined in claim 5 and arranged in a form of concentric arrays, and said second substrate is provided with a strip-shaped or line-shaped, common second electrode as defined in claim 5 and formed extending over some or all of said detecting portions belonging to one of said concentric arrays.

9. The method according to claim 5, wherein disk-shaped, said first substrate as defined in claim 5 and said second substrate as defined in claim 5 are stacked together, said first substrate carries thereon plural detecting portions as defined in claim 5 and arranged in a form of a spiral array, and said second substrate is provided with a strip-shaped or line-shaped, common second electrode as defined in claim 5 and formed extending over some or all of said detecting portions belonging to said spiral array.

10. The method according to claim 5, wherein an axis of opposition between said first electrode and said second electrode is perpendicular to a bottom wall of said reaction region.