Tagged monomers for use in sequence determination
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Abstract
A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.
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Citations
82 Claims
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- 33. A composition comprising a monomer capable of being incorporated into a growing molecular chain by a polymerizing agent, where the monomer includes a tag selected from the group consisting of a fluorescent tag, an NMR active tag, an IR active tag, an UV active tag, and a far UV active tag.
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- 40. A composition comprising a monomer including a first tag and a second tag, where the monomer is capable of being incorporated into a growing molecular chain by a polymerizing agent and at least one of the tags has a detectable property.
- 51. A composition comprising a monomer capable of being incorporated into a growing molecular chain by a polymerase, where the compound includes a first tag having a detectable property and covalently bonded to a site on the monomer that is released during incorporation of the monomer into the growing molecular chain.
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59. A composition comprising a naturally occurring or synthetic nucleotide including a tag covalently bonded to the γ
- -phosphate, where the tag has a detectable property.
- View Dependent Claims (60, 61, 65, 66)
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62. A composition comprising a naturally occurring or synthetic nucleotide including a tag covalently bonded to the γ
- -phosphate via a linker, where the tag has a detectable property.
- View Dependent Claims (63, 64)
- 67. A composition comprising a naturally occurring or synthetic nucleotide including a tag covalently bonded to the P-phosphate, where the tag has a detectable property.
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70. A composition comprising a naturally occurring or synthetic nucleotide including a molecular tag covalently bonded to the β
- -phosphate via a linker, where the molecular tag has a detectable property.
- View Dependent Claims (71, 72, 73, 74)
- 75. A modified nucleotide composition comprising a naturally occurring or synthetic nucleotide including two tags on different sites of the nucleotide, where at least one tag has a detectable property.
Specification